as quantitative real-time PCR with reverse transcription (RT–qPCR)-based tests, the gold standard for SARS-CoV-2 diagnosis, require skilled personnel and equipment infrastructure, and also have long sample-to-
Briefly, RNA samples collected from challenged mice were reverse transcribed using HiScript III RT SuperMix (Vazyme, R323-01) followed by PCR using AceQ qPCR Probe Master Mix (Vazyme, Q112-02) with 400 nM concentrations of each of the primers, as well as 200 nM of probe (GenScript...
Vazyme). Expression of interested genes were quantified using qPCR analysis on Applied Biosystems QuantStudio7 system with a standard protocol from AceQ qPCR SYBR Green PCR Master Mix Kit (#Q111, Vazyme). For
For real-time PCR analyses, mRNA levels of relative gene were measured with the 2 × AceQ Universal SYBR Green and data were normalized by GAPDH in the same samples. Primers sequences used for qPCR reactions were showed in Key resources table. ELISA Human FGF2 ELISA Kit was used to ...
The chromatin organization modifier domain (chromodomain) is an evolutionally conserved motif across eukaryotic species. The chromodomain mainly functions as a histone methyl-lysine reader to modulate gene expression, chromatin spatial conformation and g
Circular RNAs (circRNA) are a class of covalently closed single-stranded RNAs that have been implicated in cancer progression. Here we identify circNDUFB2 to be downregulated in non-small cell lung cancer (NSCLC) tissues, and to negatively correlate with