The cells were trypsinized and resuspended in phosphate-buffered saline (PBS) containing 2% FBS. Forward-scatter measurement (FSC) intensity was measured using BD FACSCalibur (BD Biosciences) and analysed with BD CellQuest Pro software (BD Biosciences). For each experiment 1,00,000–2,00,000 ce...
Filtered blocking solution of 2% FBS in PBS was added to the grids for 20 min. Immunogold labeling was performed using polyclonal antibody (pAB RBD SBF40150-T62) directed at the RBD of spike at a dilution of 1:30, followed by washing (×3) with PBS and then labeling with gold-...
打孔——预冷的甲醇methanol,4℃,20min 洗3次——PBS+2%FBS+1mM EDTA 染色 *肿瘤浸润淋巴细胞的胞内细胞因子染色【IFN-γ、Gzmb、TNF、IL-2】 细胞体外刺激+蛋白转运抑制剂——50ng/ml 佛波酯PMA{PKC激活剂}+500ng/ml 离子霉素+【2种蛋白转运抑制剂】GolgiStop(莫奈霉素){作用于蛋白在内质网的累积阶段}+...
To understand the role of SETDB1 during pluripotency regulation, we silenced Setdb1 using small interfering RNA (siRNA) and cultured ESCs under two conditions: traditional medium supplemented with 15% FBS and LIF (SL), and “ground-state” medium consisting of 2i and LIF (2iL). siSetdb1...
GP-293人胚肾细胞Human embryonic kidney cell line GP-293 DMEM+10% FBS同基布洛芬Kqtoprofqn 071-12-4 IFNA13 Protein Mouse重组小鼠IFNA13 / Ierferon alpha-13蛋白(His标签)DEPURINATIONSOLUTION去嘌呤溶液生物技术级2LRT HOS(人细胞) 5×106cells/瓶×2非01群霍乱弧菌59-43-4L-(+4℃)(剧毒)L(-)-...
ES-2人卵巢透明细胞癌ES-2 human ovarian clear cell carcinoma McCOY's 5A+10%FBS544-40-1二丁基硫96%Dibutyl sulfide 公司常年代理eBioscience品牌、Mercodia品牌、Panbio品牌、Alpco品牌、CST品牌、IBL-America品牌、AbFrontier品牌、Calbiotech品牌、Anogen品牌、Ani Biotech品牌、AAT Bioquest品牌、Jaica品牌等elisa试剂...
胎牛血清(fetal bovine serum, FBS)购自CellMax;细胞基础培养液(Dulbecco's modified Eagle medium, DMEM;含4.5 g/L D-葡萄糖,以及L-谷氨酰胺和丙酮酸钠)购自Gibco;不含EDTA的胰蛋白酶、青-链霉素溶液(100×)、D-Hanks缓冲液...
取HepG2细胞和建模成功的IR-HepG2细胞,用PBS清洗1～2次,然后每孔加入200 μL由DMEM高糖培养基(含10% FBS)配成的不同质量浓度的光甘草定(0、0.1、0.5、1.0、5.0、10.0、15.0 μg/mL),于37℃、5% CO2细胞培养箱中孵育24 h...
Lack of dystrophin causes muscle degeneration, which is exacerbated by chronic inflammation and reduced regenerative capacity of muscle stem cells in Duchenne Muscular Dystrophy (DMD). To date, glucocorticoids remain the gold standard for the treatment o
NADPH oxidase (NOX2) is responsible for reactive oxygen species (ROS) production in neutrophils and has been recognized as a key mediator in inflammatory and cardiovascular pathologies. Nevertheless, there is a lack of specific NOX2 pharmacological inhib