2.1.1亲水性成分1H-NMR样品的制备准确量取540 μL黄芪注射液与60 μL D2O(含内标TSP 0.29 mmol/L),混合均匀后转移至5 mm标准核磁管,以待分析。 2.1.2疏水性成分1H-NMR样品的制备准确量取黄芪注射液1 000 μL冷冻干燥后,加入CD3OD(含内标DMT...
Pattern recognition was applied to 1H NMR spectra from serum of patients undergoing stress test and metabolites were quantified. The effects of the stress test, confounding variables and the ability to predict ischemia were evaluated using OPLS-DA. There was an increase in lactate and alanine ...
Proton nmr parameters are reported for DMSO-d6 solutions of two receptor-selective substance P analogues: Ac[Arg6, Pro9]SP6-11, which is selective for the NK-1 (SP-P) receptor and [pGlu6, N-MePhe8]SP6-11, which selectively activates the NK-3 (SP-N) receptor. Full peak assignments...
Besides, based on previous work, further discussion was given on technical methods and key question of the 1H-NMR metabolomics method. This paper provided a methodological reference for the species identification and quality evaluation of TCM and other herbal medicines. 展开 关键词: Traditional ...
核磁共振氢谱(1HNMR)-氢原子核在磁场中的响应。利用核磁共振氢谱能够测定有机物分子中氢原子在碳骨架上的位置和数目,进而推断出有机物的碳骨架结构。 核磁共振氢谱是以样品分子中不同化学环境磁性氢原子核的吸收峰位置(化学位移)为横坐标,以测得吸收峰的相对高度(共振信号强度)...
核磁检测-核磁共振氢谱1HNMR 核磁共振氢谱 (NMR) 是一种常用的光谱技术，用于确定有机化合物的分子结构和原子间的连接方式。其中，氢谱是应用最广泛的一种，通过测量氢原子在磁场中的共振频率，可以提供有关化合物分子结构的信息。 核磁共振氢谱的原理是，将样品置于强磁场中，当用射频脉冲激发样品中的氢原子...
Herein, we demonstrate that proton-detected solid-state NMR, enabled by fast magic angle spinning (60 kHz) and high magnetic field (20 T), is feasible for bioinspired-silica-entrapped proteins. In particular, we show that basic 1H-15N correlation spectra29,40 can be acquired in fully ...
NMR found the C-terminus ordered and interacting with the active site and shown to undergo a spontaneous cleavage of the C-terminal Arg208-His209 bond that affects the product off rate. A preliminary model for the interaction based on the wild-type (WT) NmHO complexes has been presented [...
In-depth NMR studies of these methano-pyrrolidinofullerenes showed that the nitrogen inversion on the pyrrolidine moiety is not a fast event in the 1HNMR time scale as previously regarded. Solvent effects, variable temperature experiments, and protonation of the pyrrolidine nitrogen are addressed....
We review here the different methodologies used to study 1H homonuclear dipolar couplings in solid-state NMR. Because most protonated powder samples contain clusters of strongly coupled 1H nuclei, structural studies based on the measurement of individual 1H homonuclear dipolar couplings have long been ...