Now the PCR product can be sent for sequencing. In this example, the PCR product is sequenced using forward and reverse primers. Thus, two data sets, each containing a DNA sequence and a DNA chromatogram, are g
a) The expected lengths of the PCR product generated using the different primer combinations can be estimated by calculating the distance between the binding sites for the forward and the reverse primer (see Figure 2), e.g. the size of the PCR product using primer pair 8F-1492R is ~1500 ...
Bacterial identification becomes a challenge particularly in case they are either involved in an industrial process with heavy investments at risk or are a serious threat to human beings. Sequencing of the rrs (16S rDNA) of bacteria is vigorously pursued for correct identification and classification ...
DNA was isolated and sequenced using 16S rRNA primers. A length of 1452 nucleotide was sequenced and was put in public data base for obtaining accession number. The sequence was studied using MEGA 4, to estimate the evolutionary distances and to construct the Phylogenetic tree. Along with that...
Amplicon sequencing of the 1500 bp 16S rRNA gene, encompassing 9 variable regions (V1-V9), has become a powerful approach for bacterial identification [3]. Changes in gut microbiota have been linked to various metabolic disorders, such as obesity and MASLD [4,5,6]. A notable study from ...
Molecular Identification of Mycobacterium Species DNA sequences of the 26 bacterial isolates were analyzed. A phylogenetic tree was constructed to check the closeness of the sequences (Fig. 4). The sequences were also compared against Mycobacterium tuberculosis (Accession number JX303298) and M. bov...
Widely recognized as the 'gold standard' for bacterial identification due to these specific features, 16S rRNA gene sequencing has been the target of countless studies in which universal PCR primers have been applied and the resulting partial 16S gene amplicons, encompassing hypervariable regions, ...
and broad-range research analyses of mixed microbial populations. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The combination of the tw...
Rapid identification of the genus and species of bacteria in foods and clinical specimens is important. In this report, DNA sequences of bacterial 16S rDNA were used to develop the oligonucleotide array for the identification of bacterial strains of Bacillus spp., Escherichia coli, Salmonella spp....
(hypervariable) among bacterial species. The highly conserved regions allow for the design of “universal” PCR primers to target and amplify the 16S rRNA sequence, while the hypervariable regions allow for discrimination among different bacterial clades. These properties allow 16S rRNA sequencing ...