On the other hand, both R and S enantiomers of 9- and 13-HODE, 11- and 15-HETE were detected, possibly due to enzyme promiscuity rather than non-specific oxidation (by ROS or autoxidation), as confirmed by multi-loop based two-dimensional LC-MS using selective comprehensive mode wit...
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To test this, M2 macrophages co-cultured with dying adipocytes were treated with 13-HODE. Although 13-HODE did not significantly increase the adipocyte clearance rate compared to that in M2 macrophage controls, it reversed the inhibitory effect of the Alox15 inhibitor, PD146176, on phagocytosis ...
manners (Figure 3d). Similarly, upregulation of 15-LO-1 expression by SNP was in concentration- and time-dependent manners (Figure 3e). In addition, 15-LO-1 activity was examined via the measurement of 13(S)-HODE production in chondrocytes treated with tensile, H2O2, IL-1βand SNP. We...
15(S)-lipoxygenase-1 acLDL acetylated low density lipoprotein AU arbitrary unit CE cholesteryl ester ECAPCI electron capture atmospheric pressure chemical ionization FC free cholesterol 15(S)-HETE 15(S)-hydroxyeicosatetraenoic acid HODE hydroxyoctadecadienoic acid MRM multiple reaction monitoring ...
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Several studies have demonstrated that 13-HODE exerts anti-inflammatory effects as a ligand of peroxisome proliferator-acti...Takayama, H., Gimbrone Jr., M.A., Schafer, A.I., 1987. Vascular lipoxygenase activity: synthesis of 15-hydroxyeicosatetraenoic acid from arachidonic acid by blood vessels...
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arachidonic acid (5Z,8Z,11Z,14Z-eicosatetraenoic acid), linoleic acid (9Z,12Z-octadeca-9,12-dienoic acid) and chloramphenicol from Sigma Aldrich (Hamburg, Germany), HPLC standards of 5(±)-HETE, 8(±)-HETE, 11(±)-HETE, 12(±)-HETE, 13(±)-HODE, and 15(±)-HETE from Cayman Ch...