混合培养,拟讨论以下两方面问题:1)探讨不同培养时间与不同浓度13C-葡萄糖对土壤微生物群落结构的影响;2)利用13C-PLFA分析土壤微生物对13C-葡萄糖的利用情况.陈琢玉,涂成龙中国地质学会;中国矿物岩石地球化学学会第二届全国气体同位素技术及地球科学应用研讨会...
实验发现,真菌和革兰阴性菌PLFA中的13C富集速度快于革兰阳性和放线菌,但是放线菌PLFA中13C的比例随采样时间的增加而显著增加。Yuan等在水稻的不同生长期进行13CO2脉冲标记,并分析了水稻根际土壤中的PLFA,发现根际土壤中真菌PLFA中13...
磷脂脂肪酸(PLFA)是重要的生物标志物之一,能够提供有关微生物群落结构的信息.将PLFA分析与稳定性同位素13C示踪技术结合(13C-PLFA),可以指示微生物群落对于环境变化的响应及微生物群落间的相互作用.该技术在土壤生态系统的研究中已得到广泛应用.本文主要基于13C-PLFA技术的研究现状,介绍了该技术的原理和分析方法,举例...
寻标宝于2024-10-23发布土壤PLFA与13C-PLFA分析评价,项目位于陕西省,项目编号null,招标单位西北农林科技大学,中标单位宁波(北仑)中科海西产业技术创新中心。寻标宝为您提供陕西省2024年最新的招标采购信息查询服务。
(CMC) (+C), ammonium sulfate (+N), or their combination (+CN), and unfertilized soil was used as a control.13C was traced in aboveground and belowground plant biomass, soil organic matter, and microbial biomass. Microbial community composition was analyzed by phospholipid fatty acids (PLFAs...
QUANTIFICATION OF ENHANCED BIOREDUCTION OF CR(VI) USING 13C-PLFA MONITORING COUPLED WITH ADVANCED MICROBIOLOGICAL TECHNIQUESMicrobial populations increased from background levels of 200) much higher the bulk C of the labeled HRC®, reflecting the faster dissolution rate for the C-labeled polylactate ...
Yao H, Chapman SJ, Thornton B, Paterson E (2015) 13 C-PLFA: a key to open the soil microbial black box? Plant Soil 392:3–15Yao, H., Chapman, S.J., Thornton, B., Paterson, E., 2015. 13C PLFAs: a key to open the soil microbial black box? Plant. Soil 392, 3e15....
Pombo SA, Pelz O, Schroth MH, Zeyer J (2002) Field-scale C-13-labeling of phospholipid fatty acids (PLFA) and dissolved inorganic carbon: tracing acetate assimilation and mineraliza- tion in a petroleum hydrocarbon-contaminated aquifer. FEMS Microbiol Ecol 41(3):259-267...
We targeted [13C]glucose uptake by the bacterial community conditioned to a stable soil environment of 23掳C for over 2 wk rather than new bacteria generated from active colony growth caused by glucose addition. Nearly all bacterial PLFAs exhibited pronounced inhibition of13C enrichment in the ...
Dungait JAJ, Kemmitt SJ, Michallon L, Guo S, Wen Q, Brookes PC, Evershed RP (2011) Variable responses of the soil mi- crobial biomass to trace concentrations of 13C-labelled glu- cose, using 13C-PLFA analysis. Eur J Soil Sci 62:117-126...