dna-sip 13c 英文回答: DNA-SIP 13C Labeling Method. DNA-SIP (stable isotope probing) is a technique used to identify and characterize microorganisms that are actively assimilating a specific substrate. It involves incubating a microbial community with a 13C-labeled substrate, which is then ...
(2) DNA-SIP在田间和盆栽条件下都没有找到利用13C的根际微生物,而盆栽RNA-SIP的结果找到158bp,438bp所代表的细菌是根际中活跃的微生物.在研究植物-微生物... 王晋莉 - 《中国农业大学》 被引量: 2发表: 2014年 雌雄青杨对根际土壤细菌和丛枝菌根真菌的影响 (MF soil)的雌雄青杨的根际土壤细菌和丛枝菌...
13CO2标记植物地上部后,植物合成含有13C的光合作用产物并通过植物的根系和AM真菌的根外菌丝释放到土壤中被微生物所利用,并合成13C-DNA,微生物基因组总 DNA通过超高速密度梯度离心将13C-DNA 与12C-DNA 分离后, 进一步采用分子生物学技术分析13C-DNA,揭示复杂的根际和菌丝际环境中利用分泌物的活性微生物的种类和...
Probing potential microbial coupling of carbon and nitrogen cycling during decomposition of maize residue by 13C-DNA-SIP. Soil Biol Biochem. 2014; 70:12-21.Fan F, Yin C, Tang Y, Li Z, Song A, Wakelin SA, et al. Probing potential microbial coupling of carbon and nitrogen cycling during ...
C.ingentaconnectCurrent MicrobiologyNicholson, W, Fedenko, J, Schuerger, A (2009) Carbon-13 (13C) labeling of Bacillus subtilis vegetative cells and spores: suitability for DNA stable isotope probing (DNA-SIP) of spores in soils. Current Microbiology 59: pp. 9-14...
Comparative analyses of 16S rRNA sequences observed in the light and heavy fractions DNA SIP experiments incubated with 13C-glucose for 36 hrs at pH 4.5.Jeffrey S., McLeanSarah, J. FanslerPaul D., MajorsKathleen, McAteerLisa, ...