1.在拿到一个心仪的protocol后,如果在这之前没有对自己的细胞解离过,那么一定要先进行一下预实验,优化实验条件,得到一个真正适合样本、能够达到建库标准的protocol。在进行预实验的时候,需要考虑到的因素包括样本组织类型、样本来源、是否进行过基因编辑、解离时使用的培养基、选用何种酶、酶的浓度、解离时候的温度和...
1.在拿到一个心仪的protocol后,如果在这之前没有对自己的细胞解离过,那么一定要先进行一下预实验,优化实验条件,得到一个真正适合样本、能够达到建库标准的protocol。在进行预实验的时候,需要考虑到的因素包括样本组织类型、样本来源、是否进行过基因编辑、解离时使用的培养基、选用何种酶、酶的浓度、解离时候的温度和...
建议使用含有0.04% BSA (bovine serum albumin)(400 μg/ml)的 1x PBS 溶液 (无 Ca2+、Mg2+) 重悬细胞。BSA的作用是防止细胞结团,若细胞结团可适当提升BSA浓度,但最高不超过2%。 6 原代细胞和干细胞这种比较敏感的细胞类型可能需要额外的溶液或培养基。已确定以下溶液对人 293T/17和小鼠 NIH/3T3细胞系的...
This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red The complete formulation is available.Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details). 仅用于研究和生产用途。不可用于临床诊断或直接用于人类或动物...
This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red The complete formulation is available.Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details). For Research Use or Further Manufacturing. Not for diagnos...
It should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer (10X) to nine (9) parts PBS. Warning: The FOXP3 Buffer Set can have a deleterious effect on ...
and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco® PBS may require pH adjustment at the time of dilution (seeprotocolfor details).We offer a variety ofGibco® PBS formulationsfor ...
·细胞培养基及缓冲液不能含有 Ca2+和 Mg2+等影响酶活性的物质(需要用无离子的PBS进行洗涤) Q3.单细胞测序对于细胞数量有什么要求?该如何计算? 在单细胞转录组测序前期实验设计中,首先需要确定测序细胞数,在已发表的10x单细胞转录组文章中,不同文章的测序细胞数差异很大,从几千个到几万个都有可能。测序细胞数直...
This buffer is supplied as 10X PBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any sodium azide or mercury...
cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco® PBS may require pH adjustment at the time of dilution (seeprotocolfor details...