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The survivors were pooled and expanded for a few days. Genomic DNA was isolated, and inverse PCR was performed using primer sequences flanking the retroviral insertion sites followed by Illumina sequencing. The statistical significance of insertions at a given gene in the PE-treated population was...
Cell colonies that became visible were picked, expanded, and analyzed. Resulting inhibitor-resistant sublines were cultured in the presence of inhibitor at a concentration corresponding to that used during the screen. Effects of imatinib mesylate and nilotinib on Bcr-Abl autophosphorylation The ...