put the gloves inside a bucket with cold water. Do not forget to add some liquid detergent into the bucket (around 1 and ½ cup) together with a tablespoon of salt first.
The biochemical properties of proteins, such as detergent insolubility and post-translational modifications may hinder the purification and mass spectrometry detection, leading to false-negative results. Also, non-palmitoylated proteins might be co-purified with palmitoyl substrates, leading to false-...