与NuPAGE™ Bis-Tris 凝胶类似,Bolt™ Bis-Tris Plus 预制胶旨在提供一致的凝胶性能和中性的 pH 环境,从而尽量减少蛋白质修饰。Bolt 预制胶非常适合用于进行 Western 印迹转印和分析以及其他任何蛋白完整性至关重要的技术。还可使用 Bolt Bis-Tris Plus 凝胶获得理想结果,以满足日常蛋白
Invitrogen NuPAGE Bis-Tris 蛋白凝胶是预制聚丙烯酰胺凝胶,可提供宽范围分子量蛋白分离,具有高分离度并保持样品完整性。在蛋白完整性至关重要的应用中,这些预制凝胶是理想之选。与传统 Tris- 甘氨酸预制胶不同,NuPAGE Bis-Tris 预制胶具有中性 pH 环境,可尽量减少蛋白质修饰。NuPAGE Bis-Tris 凝胶适用于制备蛋白...
彩色预染蛋白分子量标准包含了从10k到180k共10种高度纯化并预染的重组蛋白质(10, 17, 25, 33, 43, 55,75, 95, 130,180k),其中75kD条带为红色,25k为蓝色和黄色条带混合物,其余条带为蓝色,10k条带在15%以下胶浓度时10k为深绿色。适合作为SDS-PAGE和Western的蛋白质分子量标准。
The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting, and may be visualized in gels or multiple times on PVDF membranes. Related Products 10x Tris/Glycine/SDS Running Buffer (1610732) 10x Tris/Glycine Running Buffer (1610734) 2x Laemmli Sample B...
Change the running buffer. TBE buffer has a higher buffering capacity than TAE. Loading buffers containing SDS may contribute to band smearing. If this occurs, use the post-staining protocol for applications requiring SDS- containing loading buffers. 2. Weak Fluorescence. The dye may have ...
纯度:Greater than 95% as determined by SDS-PAGE 应用:Western Blot, ELISA 性状:Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 溶解方法: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled ...
Resins were washed six times with PBST buffer, boiled in SDS loading buffer and analyzed by western blotting [60]. Ub-AMC assay The USP10 deubiquitinating activity assay was conducted in Ub-AMC buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mg/ml ovalbumin, 5 mM MgCl2, 1...
In the case of elution, the NTP-agarose after washing was incubated with elution buffer (assay solution in the presence of 1 mM ATP or GTP) for 30 min at 4 °C. These samples were separated with 12% SDS-PAGE and detected by Coomassie Brilliant Blue R250-staining. In silico ...
Kit Contents:Mini Gel Tank, NuPAGE Bis-Tris Mini Gels (2 Boxes, 20 Gels), NuPAGE Lds Sample Buffer (4X), NuPAGE Mes SDS Running Buffer (20X), NuPAGE Sample Reducing Agent (10X), PageRuler Plus Prestained Protein Ladder, 10 to 250 kd ...
The arrows mark the running positions of the detected isoforms, the IP antibody heavy chain, and an unidentified band (marked with question mark). F, cross-reactivity of C10.6 to Hsp60. Two-dimensional WB analysis of lysates or IPs from Jurkat (parental) cells resolved by SDS-PAGE and ...