Using Ham's F-10 Nutrient Mixture: Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to mainta...
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. 规格 细胞...
Sf9 cells were grown in GIBCO® insect culture media supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 mg/ml streptomycin. Arrb1 and Arrb2 double knockout MEFs were a gift from Dr. Robert J. Lefkowitz (Duke University). All cell lines were tested to...
RAW 264.7 (Murine Macrophages) cells and HEK-293T (Human embryonic kidney) cells were purchased from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin-streptomycin (Invitro...
UsingIMDMIMDM,amodificationofDulbecco"sModifiedEagleMedium,includesseleniumaswellasadditionalaminoacidsandvitamins.Inaddition,thisuniquemediumlacksiron,withpotassiumnitratereplacingferricnitrate.IMDMcontainsnoproteins,lipids,orgrowthfactors.Therefore,IMDMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).IMDMuses...
FBS: Fetal bovine serum IF: Immunofluorescence IGF-1: Insulin-like growth factors-1 IHC: Immunohistochemistry KEGG: Kyoto Encyclopedia of Genes and Genomes MOI: Multiple of infection NSCLC: Non-small cell lung cancer OE: Overexpression PBS: Phosphate buffered saline PH: Pleckstrin homol...
Mixed glial cell cultures were generated from the cerebral cortex of postnatal (24 h old) mice and cultured in Dulbecco’s modified Eagle’s medium/F12 containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) and an antibiotic mixture (1% penicillin/streptomycin) (Invitrogen, Carls...
The VSMCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, Zhejiang, China) and 1% Penicillin/Streptomycin (Gibco; Thermo Fisher ...
Fetal Bovine Serum?(FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2?environment to maintain physiological pH...Powder forms of Gibco? cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of pre...
AsPC-1, PANC-1, MIA PaCa-2, HPNE and HEK-293T cells were cultured in high-glucose DMEM (Gibco). All media were supplemented with 10% fetal bovine serum (FBS, Gibco), 100 IU/mL penicillin and 100 μg/mL streptomycin (NCM, Suzhou, China). All cells were cultured at 37 °C in a...