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准备10%FBS DMEM/F12 (Corning Cat.No.10-092-CV),准备0.1%BSA 的冰PBS及Y27632(10mM); 向15ml离心管中预先加入5ml平衡至室温的含10%FBS的DMEM/F12 ,各离心管提前标记复苏的类器官; 从液氮罐中取出冻存管,37℃水浴锅解冻,解冻时间1-2分钟,最佳解冻状...
Cells were incubated with 2 µM Fluo-3-AM in RPMI supplemented with 10% FBS for 30 min at 37 °C in the dark, washed in PBS and seeded on 35-mm glass-base dishes with Ca2+ imaging buffer (120 mM NaCl, 5 mM KCl, 0.96 mM NaH2PO4, 1 mM MgCl2, 11.1 mM ...
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After washing with FACS buffer (PBS with 2% FBS (Sigma Aldrich)), the samples were acquired using a LSRFortessa flow cytometer (BD Biosciences) and the FACSDiva software. The data were analyzed with FlowJo version 10 (BD Biosciences). Quantitative RT-PCR Total RNA extraction from SKNMC SAM ...
MFC小鼠细胞 MFC mouse gasic cancer cells RPMI-1640(GIBCO)+10%FBS(肼基羰基)二茂铁(>95.0%(HPLC)(T),用于高效液相色谱标记)质量规格:>95.0%(HPLC)(T),用于高效液相色谱标记(Hydrazinocarbonyl)ferrocene IL36RN Protein Human 重组 IL1F5 / IL36RN 蛋白二甲亚砜(>99.0%(GC))质量规格:>99.0%(GC)Dimet...
大鼠膀胱成纤维细胞价格:￥2550/株产品详情:使用方法:1. 贴壁细胞的消化:a) 吸去培养液,用无菌的PBS、Hanks液或无血清培养液洗涤细胞一次,以去除残余的血清。b) 加入少... 纯度:详见说明书% 包装规格:详见说明书 用途:公司产品仅用于科研 货号:FS-X6567 ...
SVEC4-10(小鼠淋巴结内皮细胞)是来源于成年小鼠腋窝淋巴结的上皮细胞样贴壁细胞系,不含真菌、细菌、HIV、支原体、衣原体等污染,生长培养基:DMEM+10%FBS+1%P/S。 SVEC4-10(小鼠淋巴结内皮细胞) SVEC4-10(小鼠淋巴结内皮细胞)复苏操作: 1)将培养基在37℃水浴锅预热;准备一个15mL无菌的离心管,加入8mL预热...
(PBS). After an incubation for 20 minutes at 37 °C, the cells were collected by centrifugation in a swinging bucket at 100 gfor 5 minutes. The supernatant was removed and Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies), supplemented with 10% FBS (Thermo Fisher ...