This invention relates to new polypeptides having a 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity, which polypeptides comprise a sequence having at least 90 % identity with the sequence SEQ ID N掳 1 or fragments thereof. These polypeptides are responsible of a massive accumulation of ...
, Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches, DOI 10.1007/978-1-4614-4063-5_2, © Springer Science+Business Media New York 2013 2 1-Deoxy- D -Xylulose 5-Phosphate Synthase (DXS), a Crucial Enzyme for Isoprenoids Biosynthesis Song Xiang , ...
1). The natural substrate for this reaction, 1-deoxy-d-xylulose 5-phosphate (DXP),4 is generated from pyruvate and d-glyceraldehyde 3-phosphate (d-GAP) in a thiamine diphosphate (ThDP)-dependent reaction catalyzed by DXP synthase (5). This first step is believed to be rate-limiting in ...
EC Number 2.2.1.7 Systematic name pyruvate: d -glyceraldehyde- 3-phosphate acetaldehydetransferase (decarboxylating) Recommended name 1-deoxy-D-xylulose-5-phosphate synthase Synonyms 1- d -deoxy- d -threo-2-pentulose 5-phosphate synthetase 1- d -deoxyxylulose 5-phosphate synthase 1-deoxy- d -...
The initial step of the plastidic 2C-methyl-d-erythritol 4-phosphate (MEP) pathway that produces isopentenyl diphosphate is catalyzed by 1-deoxy-d-xylulose-5-phosphate synthase. To investigate whether or not 1-deoxy-d-xylulose-5-phosphate synthase catalyzes a limiting step in the MEP pathway ...
In the non-mevalonate pathway (MEP), the 1-deoxy-D-xylulose-5-phosphate synthase 1 (DXS1) enzyme has been postulated to catalyze the rate- limiting step in the formation of plastidial isoprenoids. In tomato, the function of DXS1 has only been studied in fruits, and hence its functional ...
The development of drug resistance by Mycobacterium tuberculosis and other pathogenic bacteria emphasizes the need for new antibiotics. Unlike animals, most bacteria synthesize isoprenoid precursors through the MEP pathway. 1-Deoxy-d-xylulose 5-phosphate
1-deoxy-D-xylulose 5-phosphate synthase(DXS) catalyzes the initial step of the 2-C-methyl-D- erythritol 4-phosphate(MEP) pathway consisting in the condensation of (hydroxiethyl)thiamin derivedfrom pyruvate with D-glyceraldehyde 3-phosphate (GAP) to yield1-deoxy-D-xylulose 5-phosphate (DXP)....
Andrographis paniculata 1-deoxy-D-xylulose-5-phosphate synthase (ApDXS) gene (GenBank Accession No MG271749.1) was isolated and cloned from leaves for the first time. Expression of ApDXS gene was carried out in Escherichia coli Rosetta cells. Tissue-specific ApDXS gene expression by quantitative...
Use of a DNA sequence encoding 1-deoxy-D-xylulose-5-phosphate synthase (DOXS), and optionally p-hydroxyphenylpyruvate dioxygenase (HPPD) and/or geranylgeranyl-pyrophosphate oxidoreductase (GGPPOR), to produce a plant with increased tocopherol, vitamin K, chlorophyll and/or carotenoid content is new...