For the mutant assay, HIF-2α™ plasmid and mHIF-2α S304M + G323E plasmid were used. For theCpt1arHRE binding assay, the pGL3 basic vector (Promega) was cloned with the presumed rHRE1 region in theCpt1a
Interestingly EGF stimulation of cells expressing empty vector or FLAG-Bα/δ subunits produced a subtle upward shift in the migration of Raf1 on SDS-polyacrylamide gels (Fig. 8B, top panel). Because PP2A ABαC and ABδC appear to be recruited to Raf1 following EGF treatment, the slower ...
The mutants were cloned into the mammalian expression vector pCB6. For complementation studies in yeast, mutants were introduced in the yeast expression vector pVT. Table 1. Primers used for mutagenesis PrimerNucleotide sequence, 5′ to 3′Position in Nramp2 pCB6 F aatgggcggtaggcgtgta 848-866...
(a) MCF10A cells were infected either with a retroviral control vector (Mock) or a retrovirus encoding a constitutively active form of ERBB2 (NeuT). Following puromycin selection, cells were grown in the presence or absence of EGF during 48 h and FLIPL, BimEL, ERK1/2, c-myc, ERBB2 ...
Script Event v3.0 < Vector Method | Stat Method | Moist Method | Bit Method > Parachute Model Crash Lester Cummy WindSock Crash (This will almost Certainly Kill The Entire Session!) Use Ruiner Crash Test Crash (Teleports Player to Kill Barrier and trys force their character to Control Their...
imageVector = Icons.Default.Home, contentDescription = "홈 아이콘", tint = Color.White, modifier = Modifier.size(30.dp) ) } IconButton(onClick = {}) { Icon( imageVector = Icons.Default.Search, contentDescription = "검색 아이콘", tint = Color.White, mod...
Let’s consider the first word, i.e., “When” and calculate the positional embedding vector for it.让我们考虑第一个单词,即“When”,并计算它的位置嵌入向量。 There are two formulas for positional embedding:位置嵌入有两个公式: The POS value for the first word, “When” will be zero since...
The matrix has eigenvalue 1 with eigenvector (pmatrix) 0 1(pmatrix) so alpoints on the y-axis are invariant. The other eigenvector is (pmatrix)11(pmatrix) so all lines parallel to y=x stay parallel to y=x under T .Since every line will cross the y-axis at one point, and ...
Comparative analysis of in vivo and in vitro AAV vector transduction in the neonatal mouse retina: effects of serotype and site of administration. Vision Res 2008; 48: 377–385. 31. Back SH, Scheuner D, Han J, Song B, Ribick M, Wang J et al. Translation attenuation through eIF2alpha...
and a ‘GSAS’ substitution of the furin cleavage site (682–685 amino acids (aa) in WA1) were introduced into the spike-expressing plasmids35, and then the ectodomain (1–1,208 aa in WA1) of the spike was fused with a C-terminal 8× His-tag and cloned into the paH vector. ...