I mean, I think the cause of the Civil War was basically how government was going to run.我的意思是,我认为内战的原因基本上是政府的运作。The freedoms and what people could and couldn't do.自由权,人们能做什么,不能做什么。In the year 2023, it's astonishing to me that you answered th...
Knockdown or pharmacological inhibition of SOD1 has been shown to effectively suppress the growth and proliferation of lung cancer cells and xenograft tumors7,10,15,16,17. However, the role of SOD1 in lung cancer has not been investigated genetically. We, therefore, generated TAM-inducible whole...
The cells and tissue sections were fixed in 4% paraformaldehyde for 20 min, washed three times in PBS-T (PBS with 0.1% Triton X-100) and permeabilized for 15 min using 0.3% Triton X-100. Blocking was performed for 1 h in 5% goat serum and the samples were incubated with ...
studies, at three weeks after boost, six mice from each group were anesthetized with CO2 and then infected with the minimal level of five times the 50% mouse lethal dose (5 × MLD50) of the mouse-adapted re-assortant influenza A H3N2 virus strain A/Hong Kong/1968 in PBS via the i....
The inner layer of the retina was separated and soaked in 0.15 mol/L pH 7.4 glycine buffer overnight after being rinsed with PBS. Then, it was digested with 3% trypsin at 37 °C for 2 h. After the retina was dissolved, it was gently shaken in distilled water to wash away the inner...
Gel filtration chromatography was performed as described previously (Gusmaroli et al., 2004) except that the column was equilibrated with PBS containing 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4. For the immunoblot analysis, fractions 9 to 21 were concentrated using acetone...
The cells were then washed twice with high-glucose DMEM and twice with PBS, and added to serum-free and phenol red-free high-glucose DMEM with compounds in different concentrations and incubated for 24 h at 37 °C. After this incubation, glucose content in the culture medium was measured ...
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The precipitants were washed three times by PBS containing 0.1% NP-40 and then subjected to SDS-Page. Antibodies against DDX58 (sc-376845), ADAR1 (CST #14175S), STAT1 (CST #9172), phospho-STAT1 (Y701) (YP0249), OVA (Zen Bioscience #220115), PSMB4 (sc-390878), PSMB8 (sc-...
Seeking and identifying time window of antibiotic treatment underin vivoguidance of PbS QDs clustered microspheres based NIR-II fluorescence imaging Sijia Feng, Mo Chen, Yuzhou Chen, Liman Sai, ... Jun Chen Article 138584 Chemical Reaction Engineering ...