Gibco™ 胰蛋白酶 1:250 粉由来自猪胰腺的蛋白酶辐照混合物制成。鉴于其酶解强度,其在常规细胞培养传代和初生组织解离中被广泛用于细胞解离。我们提供多种 Gibco™ 胰蛋白酶和非动物源性 TrypLE™,特点为:•质量检测•可追溯性记录•了解更多信息 ...
Cells incubated only with HBSS, containing 10 mM HEPES pH7.4, at 37 °C for 8 h were harvested with trypsin and counted, for control. Staurosporine (2 μM) treatment was used as positive control for cell death, and was incubated in the same conditions above for 24 h. Cell ...
For isolation of neutrophils, bone marrow cells derived from 6 to 8-week-old BALB/c mice were harvested in Hank’s buffered salt solution (HBSS; Gibco), subsequently filtered through 40-μm nylon mesh and added to the top of a 2-layer Percoll (GE Healthcare) gradient (72% and 63.5% ...
For mitochondrial mass measurements, cells were stained with 50 nM MitoSpy Green (BioLegend) or 50 nM MitoTracker Deep Red (ThermoFisher Scientific) in HBSS at 37 °C for 20 min, followed by viability and surface marker staining as described above. Apotracker Green (BioLegend) was used at...
The Wharton’s jelly tissues were washed multiple times with HBSS to remove excess blood and were subsequently cut into small pieces. The pieces were transferred to 10 cm2 dishes with 5 mL of minimum Eagle’s medium alpha (Gibco) containing 10% fetal bovine serum (FBS, Gibco) and 1% ...
Cells in a 6-well plate at an appropriate density were sequentially incubated in Hanks’ balanced salt solution (HBSS) containing 5 µM MitoSOX™ Red mitochondrial superoxide indicator (Invitrogen, USA) for 10 min at 37 °C, washed gently with PBS three times, preserved in preheated HBSS...
The dye was removed and 1 mL of HBSS with 20 mM HEPES, pH 7.4 (Thermo, 14170112) was added to each dish for 10 min prior to microscopy. A brightfield image was taken prior to cell stimulation and imaging. Fluo-4 microscopy was performed using Spinning Disk Confocal microscopy at 40X ...
The cell responses with the pre-treatment with the HBSS only were also included as control. Full size image Discussion There is growing evidence that GPCR signaling is complicated – many GPCRs including PAR2 elicit both G protein-dependent and independent signaling events [20, 31]. To ...
PBMC or U1 cells were incubated in flow buffer (HBSS + 2% FBS + 0.05% sodium azide) with primary antibody for 30 min at 4°C. Cells were then washed with flow buffer and incubated in flow buffer with the secondary antibody (Biotin-conjugated goat anti-mouse IgG; Jackson ImmunoResearch ...
empty vector cells.The accumulation amount of MPP +in the developed MDCK-hOCT1，hOCT1P341L and hOCT1M420del cells were 12.5，13.7and 12.0times of those of MDCK empty vector cells ，and the inhibitor TEA +could significantly reduce the accumulation.CON-CLUSION The developed MDCK-hOCT1，...