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When the cell density reached about 2 × 106 cells mL−1, 120 mL high-titer virus stocks, 1 μM alprenolol (Sigma-Aldrich), and 14 μM E-64 (Peptide Institute, Inc.) were added, and the culture was continued. Cells were harvested 48 h post-infection by centrifugation at...
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www.nature.com/scientificreports OPEN Optimization of an O 2‑balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment Anne Mouré1,8, Sawsen Bekir1,8, Elodie Bacou1, Quentin Pruvost1, Karine Haurogné1, Marie Allard1, Laurence De...
Intracellular acidification has been shown to func- tionally uncouple STIM1 and Orai1 without causing a complete dissociation of STIM1/Orai1 complex, suggesting 1School of Medical Sciences, University of Adelaide, Adelaide, South Australia, 5005, Australia. 2School of Medicine, University of ...