Genome copies per µl RNA template were calculated based on a quantified standard RNA, where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The limit of detection was ...
一、项目基本情况项目编号:NNZC2024-G1-991086-GXZL项目名称:重点传染病及污水监测相关试剂耗材采购项目(重)预算金额:443826.00元;最高限价:443826.00元;采购需求:序号标的的名称数量及单位简要技术需求或者服务要求1ddPCR™SupermixforProbes(NodUTP)1盒具体内容和数量以招标文件第二章《采购需求》为准。21-StepRT...
The relative expression of the mutant and wild-type alleles was determined using 2-step RT–PCR. Primers were designed to target exons 4 and 5 of theMLLT1gene (NM_ 005934). Complementary DNA was synthesized using the SuperScript VILO cDNA kit from Invitrogen. The manufacture protocol was foll...
The relative expression of the mutant and wild-type alleles was determined using 2-step RT–PCR. Primers were designed to target exons 4 and 5 of the MLLT1 gene (NM_ 005934). Complementary DNA was synthesized using the SuperScript VILO cDNA kit from Invitrogen. The manufacture protocol was ...