It is well known that the ATP-Mg complex, in the range of millimolar concentration, is the largest pool of Mg2+ions within the cell. The complex follows a 1:1 stoichiometry, with aKdvalue of 0.15 mM23. Since ALDH1A3 activity is activated by free Mg2+, it is conceivable that it a...
AtMRS2-10 might not be high enough, it should be noted that Al ions have strong affinity for biomembranes [32] and bind almost 107 times more tightly to ATP than Mg2+ [33], which means that aluminum concentrations less than nanomolar are required to compete with millimolar Mg concentrations...
Thus, in vitro, micromolar concentrations of EN460 can inhibit ERO1α even in the presence of a vast molar excess of competing thiols. In vivo, nonselective reactivity with free thiols may explain the leftward shift in the dose-response curve (compared with the in vitro situation). ...
M molar or molarity m multiplet Me methyl mesyl methanesulfonyl mg milligram(s) MHz megahertz (frequency) Min minute(s) mL milliliter(s) mM millimolar mmol millimole(s) mol mole(s) MS Mass spectral analysis MTBE methyl t-butyl ether N normal or normality NBS N-bromosuccinimide NCS N-chlor...
It had a specific activity of about 60 to 70 units (micromoles NO2 per minute per milligram protein). A sequential elution with NADH followed by KNO3 (0.3 molar) or KCl (0.3 molar) yielded 2 peaks. Rechromatography of each peak gave two peaks again. These results indicate that we are ...
“μM” means micromolar, “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmole” mean micromole(s), “g” means gram(s), “μg” means microgram(s), “ng” means nanogram(s), “U” means unit(s), “bp” means base pair(s), and “kB” means ...
Lipids in stock solutions in chloroform were mixed at the desired molar ratio, and the solvent was evaporated. The lipid film was hydrated in appropriate buffer. The lipid suspension was incubated at room temperature for 30 min and extruded 20 times through polycarbonate 0.1-mm filter using a ...
Glycerate 3-phosphate (20 millomolar) slowed the initial rate but not the extent of activation and rubisco activase had no effect on this. The activation of rubisco was shown to be affected by phosphoenolpyruvate (3 millimolar) but not by creatine phosphate (3 millimolar) or ATP (3 millimolar...
For western blot analysis, the liver tissues were homogenized using a SuperFastPrep-2 using MP Biomedicals Matrix D Beads at the 6 setting for 40 s in radioimmunoprecipitation assay (RIPA) lysis buffer (1 molar (M)) sodium chloride, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium ...
Because the lanthanide cations feature rather low molar absorption, phen molecules apparently act as photosensitizers (“antennas”), which then transfer the absorbed energy to the emitting Ln3+ cations by a dipole–dipole energy transfer mechanism [51]. Such energy transfer is known to be quite ...