Thus, in vitro, micromolar concentrations of EN460 can inhibit ERO1α even in the presence of a vast molar excess of competing thiols. In vivo, nonselective reactivity with free thiols may explain the leftward shift in the dose-response curve (compared with the in vitro situation). ...
Unilamellar liposomes were prepared by the freeze-thaw and extrusion method using dioleoylphosphatidylcholine and dioleoylphosphatidylserine at a molar ratio of 9:1 [22]. Briefly, the lipid film was suspended in 20 mM HEPES buffer at pH 7.0 with 100 mM KCl; 50 μΜ EDTA; and either...
M molar or molarity m multiplet Me methyl mesyl methanesulfonyl mg milligram(s) MHz megahertz (frequency) Min minute(s) mL milliliter(s) mM millimolar mmol millimole(s) mol mole(s) MS Mass spectral analysis MTBE methyl t-butyl ether N normal or normality NBS N-bromosuccinimide NCS N-chlor...
Glycerate 3-phosphate (20 millomolar) slowed the initial rate but not the extent of activation and rubisco activase had no effect on this. The activation of rubisco was shown to be affected by phosphoenolpyruvate (3 millimolar) but not by creatine phosphate (3 millimolar) or ATP (3 millimolar...
“μM” means micromolar, “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmole” mean micromole(s), “g” means gram(s), “μg” means microgram(s), “ng” means nanogram(s), “U” means unit(s), “bp” means base pair(s), and “kB” means ...
The enzyme was eluted from the blue-sepharose column with a linear gradient of NADH (0-100 micromolar) or with 0.3 molar KNO3. About 10% of the original activity was recovered with NADH (NADH-NR). It had a specific activity of about 60 to 70 units (micromoles NO2 per minute per ...
molar absorption coefficient (ε370 = 29,500 M−1·cm−1). The enzymatic reaction was carried out for 15 min at 37 °C in a final volume of 500 µL, after 5-min preincubation with 2 mM, 5 mM and 7.5 mM of each ATP, ADP or AMP, and using 50 mM ...
Lipids in stock solutions in chloroform were mixed at the desired molar ratio, and the solvent was evaporated. The lipid film was hydrated in appropriate buffer. The lipid suspension was incubated at room temperature for 30 min and extruded 20 times through polycarbonate 0.1-mm filter using a ...
For western blot analysis, the liver tissues were homogenized using a SuperFastPrep-2 using MP Biomedicals Matrix D Beads at the 6 setting for 40 s in radioimmunoprecipitation assay (RIPA) lysis buffer (1 molar (M)) sodium chloride, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium ...
A strong contribution of the red component of Eu3+ is clearly observed, as the sample 4, containing only 0.1 molar percent of europium(III) dopant in the yttrium(III) matrix, appears on the color diagram nearly in the middle between the pure Y3+ (1) and Eu3+ (2) samples, ...