A solution of the isourea 3 (2.78 g; 5.41 mmol) and benzyl bromide (1.93 mL; 16.2 mmol) in acetonitrile (15 mL) was refluxed overnight. The reaction was evaporated and subjected to column chromatography (flash SiO2; 20% EtOAc/hexane) to give 3.02 g of 4 as a white foam (95% yield...
. Data shown as % of input normalized to vehicle in the C/EBPδ- or C/EBPβ-pulldown.hWestern blot on protein collected 30 min after adipogenic induction with IL-1β and indicated inhibitor(s) (one representative blot from 3 independent experiments).i–kGene expression (i,j) and lipi...
(etwa 2200 Ci/mmol, New England Nuclear, Boston, MA, USA) mit und ohne 0,05 ml nicht-markierten kompetitiven Testpeptiden inkubiert. Nach 100 Minuten Inkubieren (25°C) wurde das gebundene (125I)GLP-1(7-36) von dem freien durch rasche Filtration durch GF/C-Filter (Brandel, ...
(B) Jurkat cells were transfected with the 1.2 kb FasL promoter reporter construct (20 μg) plus or minus expression constructs for IκB or IκBαM (20 μg). Cells were then treated with etoposide (5 μM) or teniposide (5 μM), and reporter activity was determined after 12–16 hr...
HMECs (Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in MCDB131 medium (Gibco) containing 10% FBS, 1 μg·mL−1 hydrocortisone, 2 mmol·L−1 L-glutamine, and 10 ng·mL−1 epidermal growth factor. Cells were maintained at 37 °C in a 5%...
In another 100 mL two-necked flask (flask 2), 7 g (25.17 mmol) of FeSO4¨7H2O were dissolved in 35 mL of degassed water. After the complete dissolution of ferrous sulphate, the degassed aqueous solution of (NH4)2(C6H5PO3) was transferred from flask 1 to flask 2 under a stream of...
The membrane was blocked with TBST (150 mmol/L NaCl, 50 mmol/L Tris-HCl, pH 7.4, 0.1% Tween 20) containing 5% dry milk for 1 hour and primary antibodies were added at a 1:1000 dilution overnight at 4°C. Horseradish peroxidase-conjugated anti-rabbit IgG (DAKO Ltd., Cambridge, UK)...
In the fasted state, the young lean rats had low blood glucose, about 3.3 mmol/L. The intermediate and old rats had much higher blood glucose, about 6.0 mmol/L (Figure 8A). In the fed state the three groups had similar blood glucose levels, around 8 mmol/L. The fed/fasted...
Digestible energy is defined as: a. None of these. b. Gross energy minus fecal energy minus energy in urine and gaseous waste. c. Gross energy minus energy in feces. d. Energy in feces and urine. Haustrum: a) Large intestine b) Small intestine...
Maternal Placental Blood Flow Is Reduced in Proportion to Reduction in Uterine Driving pressure1 MARY R. LAIRD, J. JOB FABER, AND NANCY D. BINDER Departments of Pediatrics and Physiology, Oregon Health Sciences University, Portland, Oregon 97201-3098 The relationship between uterine driving pressure...