上海玉博生物科技有限公司 主要致力于“2-Mercaptoethanol, 10 x 1 mL Ampules pierce-35602”的生产销售。多年的“2-Mercaptoethanol, 10 x 1 mL Ampules pierce-35602”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户
CH12 cells were cultured in RPMI 1640 supplemented with 12% FBS (Sigma F6178), penicillin (100 U/ml)/streptomycin (100 μg/ml), 50 μM 2-mercaptoethanol, 1x MEM nonessential amino acids (Gibco 12084947), 1 mM sodium pyruvate (Gibco 11530396), and 10 mM HEPES (Gibco 11560496...
1) 准备DMEM培养基;北美胎牛血清,10%;2 mM glutamine(谷an酰胺);1 mM pyruvate(丙酮酸盐); 50 μM 2-mercaptoethanol(巯基乙醇);双抗,1%。 2) 培养条件: 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 3) 冻存细胞:冻存细胞时,用FBS重悬细胞,然后加入一定量的DMSO,轻轻混合均...
试剂:1640培养液、缓冲液、PBS、消化液、血清、抗生素、2-巯基乙醇。 THP-1细胞培养条件 THP-1细胞培养基:90% 1640+10% FBS+PS+ 0.05 mM 2-巯基乙醇(2-mercaptoethanol) THP-1细胞传代比例:1:2至1:3,每周 2-3次 THP-1细胞代次密度:如果THP-1细胞密度达80%-90%,即可进行传代培养 生长条件:气相:95%...
used in the reaction was: 25 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 2 mM 2-Mercaptoethanol, 0.15 mM Biotin, 2 mM ATP. Biotinylated proteins were purified by size-exclusion chromatography in 20 mM citric acid pH5, 150 mM NaCl and subsequently used in GCI ...
Physical form Solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 10 mM 2-mercaptoethanol, 10 μM 4-2-aminoethylbenzenesulfonyl fluoride, 0.01% leupeptin 计算化学 1.疏水参数计算参考值(XlogP):无 2.氢键供体数量:4 ...
Physical form Solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 10 mM 2-mercaptoethanol, 10 μM 4-2-aminoethylbenzenesulfonyl fluoride, 0.01% leupeptin 存储温度:−70°C 安全信息我要纠错 安全说明编码 :22-24/25危险品运输编号:NONH for all modes of transportWGK Germ...
THP1 cells (Ubigene) were grown in RPMI 1640 supplemented with 0.05 mM 2-mercaptoethanol (Sigma). All cells were incubated at 37 °C with 5% CO2 in medium containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin solution (Beyotime) to ensure optimal growth conditions. ...
TargetMol1ml99.17%;1 mL * 10 mM (in DMSO)￥240.00￥192.00购买 Acmec20mg分析对照品,GC≥98%￥199.00￥149.00购买 alpha-三联噻吩用途 2,2':5',2''-Terthiophene (α-Terthiophene) 是杂环噻吩的低聚物,2,2':5',2''-Terthiophene 通常作为有机合成砌块 。
(GE Healthcare) and incubated in the dark for 5 h. Excess dye was removed by dialysis and refolded with wild type H3 in refolding buffer (20 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol). Refolded H3–H4 was purified over a Superdex200 increase column ...