10XBuffer Tango 到1XBuffer Tango稀释10倍；10XBuffer Tango 到2XBuffer Tango稀释5倍；以20ul体系算，如果要求1XBuffer，就加2ul 10XBuffer，如要球2XBuffer，就加4ul 10XBuffer
10XBuffer Tango 到1XBuffer Tango稀释10倍; 10XBuffer Tango 到2XBuffer Tango稀释5倍; 以20ul体系算,如果要求1XBuffer,就加2ul 10XBuffer,如要球2XBuffer,就加4ul 10XBuffer 分析总结。 以20ul体系算如果要求1xbuffer就加2ul10xbuffer如要球2xbuffer就加4ul10xbuffer结果...
答案解析 查看更多优质解析 解答一 举报 10XBuffer Tango 到1XBuffer Tango稀释10倍;10XBuffer Tango 到2XBuffer Tango稀释5倍;以20ul体系算,如果要求1XBuffer,就加2ul 10XBuffer,如要球2XBuffer,就加4ul 10XBuffer 解析看不懂?免费查看同类题视频解析查看解答 更多答案(1) ...
MBI BY5 10X Buffer Tango™ 5x1ml 130 MBI BO5 10X Buffer O 5x1ml 130 MBI BR5 10X Buffer R 5x1ml 130 MBI EF0221 Pyrophosphatase, Inorganic (from yeast) 10units 310 MBI EF0261 Bacterial Alkaline Phosphatase 7500units 410 MBI EF0341 Calf Intestine Alkaline Phosphatase 200units 120 MBI EF...
设置两个反应体系,均加入Xho 和EcoRI各3 gL,10xbufferTango,其中一体系加入399pEGFP质粒,另一体 浴2.5h。两体系产物分别进行0.8%琼脂糖凝胶电泳,割胶回收目的基因片段(400 ng)及线性化的pEGFP质粒(200ng),将产物中加入2此T4连接酶,10xbuffer min。将连接后的产物 2.5此,灭菌水补充体积至25此,于22℃水浴中...
酶切体系为:pART-CAMvector 1μg; XbaI 2μL;XhoI 1μL;10xTango buffer 4μL;无菌ddH 2 O补足至20μL;30℃反应1.5h,37℃反应1.5h,65℃,20min。酶切结束后,根据Takara琼脂糖凝胶回收试剂盒回收载体大片段。 利用无缝克隆技术(In-fusion HD Cloning Kit,Takara)构建pART-CAM::LrPAL-1过表达载体。
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit ...
IOXBuffer tango 10 μ I pEGFP-DCFl 2 μ I Ase II μ I 将配制好的上述体系放入37°C孵育器中,反应时间为3小时左右。 [0017]碱性磷酸酶脱磷处理: 将上述酶切产物,经割胶回收,稍浓缩后,配制脱磷体系。 [0018]ddH202μ I FastAP I μ I IOXBuffer 2μ IpEGFP-DCFl 15 μ I ...
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Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 背景介绍 PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a mul...