1 Microgram of RNA was reverse transcribed at 37 °C for 120 min using primers as described below using Omniscript Reverse Transcription Kit (QIAGEN). cDNA obtained from the reverse transcriptase (RT) reaction (amount corresponding to 1 µg of total RNA) was subjected to PCR using Quanti...
which are a highly tumorigenic and self-renewing sub-population of tumour cells that have different functional characteristics1,2. As the self-renewing capability and multipotency may be closely associated with tumour progression
Nuclear import of the HIV-1 reverse transcription complex (RTC) is critical for infection of non dividing cells, and importin 7 (imp7) has been implicated in this process. To further characterize the function of imp7 in HIV-1 replication we generated cel