Conclusion As the upstream regulatory gene of SERPINE1, miR-532-3p inhibits the proliferation, migration and invasion of colon adenocarcinoma cells by regulating ECM RECEPTOR INTERACTION. MiR-532-3p/SERPINE1 has the potential to become a new target for the treatment of colon adenocarcinoma. 【Key...
10 Sudan black B staining of recombinant yeast cells 两个向日葵 LACS 基因与拟南芥 AtLACS8 和 AtLACS9 的相似性较高,其中向日葵 HaLACS8 蛋白定位于 内质网,HaLACS9 蛋白定位于叶绿体. 并且研究 表明,位于不同细胞器中的 LACS 蛋白具有不同的 生物学功能.位于质体中的 LACS 蛋白与质体中从 头合成的长链...
Treatments with IL-1RN and the IL-1β monoclonal antibody canakinumab reduce the expansion of leukemic cells, including CD34+ progenitors, in AML xenografts. In vivo deletion of IL-1rn induces hematopoietic stem cell (HSC) differentiation into the myeloid lineage and hampers B cell development ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
To analyze the clinical features and genotype in a 8-year-old boy with dyskeratosis congenita (DC). Methods We reviewed the clinical data of the case and amplified 7 DC-related genes (including DKC1, TERT, TERC, TINF2, NOP10, NHP2 and WRAP53) using polymerase chain reaction for DNA ...
Compared to control spheroids, the residual spheroids were larger upon PHD inhibition and contained more cells with tight VE-cadherin positive cell-cell contacts. Morphological alterations were dependent on stabilization of HIF-1α and not HIF-2α as shown in cells with stable knockdown of HIF-α...
To further analyze the amplicon, the purified fragment was cloned into pGEM-T Easy vector (Promega), the detail of indels (insert/delete) were identified and compared with control. Luciferase activity assay and p24 gag detection by ELISA The modified cells were infected with R5-tropic HIV-1YU...
Although complications and clinical symptoms of COVID-19 have been elucidated, the prevalence of long-term sequelae of COVID-19 is less clear in previously hospitalized COVID-19 patients. This review and meta-analysis present the occurrence of different symptoms up to 1 year of follow-up for ...
In particular the aims of this work were: 1) to analyze the phylogenetic distribution of CeoB-like pumps in the Burkholderia genus; 2) to define the function(s) they perform within the Burkholderia genus and 3) to try tracing the evolutionary history of these genes in Burkholderia. ...
首先,打开Mega程序,有如下图所示的操作界面: Fig 单击工具栏中的“File”按钮,会出现如下图所示的菜单: Fig 从上图可以看出,下拉菜单有“Open Data”(打开数据)、“Reopen Data”(打开曾经打开的数据,一般会保留新近打开的几个数据)、“Close Data”(关闭数据)、“Export Data”(导出数据)、“Conver To MEGA...