A 3-µl volume of cDNA (1:20 dilution) was used in a 10 µl quantitative-PCR reaction using SsoAdvanced Universal SYBR Green Supermix (BioRad, cat no. 1725271) and 250 nM primers (Supplementary Table 1). Parallel tbb-2 quantitative PCR reactions were run for each sample for ...
The slides were immersed in 70% ethanol for 5 min, dried at 37 °C for 15 min and then stained in SYBR Gold nucleic acid gel stain (Invitrogen, S11494) for 30 min in the dark. Analysis of neutral comet assay plus radiation was performed 24 h after 5 Gy ionizing ...
Finally, real-time quantitative PCR (RT-qPCR) was carried out using the SYBR Green qPCR Master Mix (Applied Biosystems). mRNA expression values were calculated using the ΔΔCt method and GAPDH gene as a control. A detailed list with primers used in the present study is provided in Table ...
Oligonucleotide products were then resolved on a 2% agarose gel and visualized after staining with Sybr Gold (Invitrogen, Carlsbad, CA). The wild-type allele resulted in amplification of a 1300-bp product and the null allele a 600-bp product [55]. Histological analysis of circumvallate ...
Quantitative real-time RT-PCR analysis was performed using SYBR® Fast qPCR Mix (TaKaRa, Shiga, Japan) and a Agilent Technologies Stratagene Mx3000p Real-Time System (Agilent, USA) with SYBR Green to determine the mRNA expression level of a gene of interest. A reference gene, glyceraldehyde...
RT-qPCR assays consisting of three biological and three technical replicates were performed on a CFX96 (Bio–Rad) using the SYBRGreen PCR Supermix kit (Bio–Rad Laboratories). Banana MaRPS476 and tomato SlActin77 genes were adopted as the reference genes. Transcript levels were calculated ...
Particle-associated RT activity was determined by SYBR Green-based Product-Enhanced Reverse Transcription assay (SG-PERT) (Pizzato et al., 2009). To produce AAV vectors, HEK293T cells grown on 15-cm dishes were transfected using polyethylenimine (PEI) (Merck), a transfection reagent for ...
Detection MethodSYBR FormatTube PCR Method1-step RT-qPCR PolymeraseAmpliTaq Gold DNA Polymerase Reaction SpeedStandard For Use With (Equipment)7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, Step...
(学院),universityBR_TheU.S.GradeSchoolSyBR_GradeSchoolSystem_picBR_GradeSchoolSystem_piBR_GradeSchoolSystem_picBR_GradeSchoolSystem_piBR_GradeSchoolSystem_picBR_GradeSchoolSystem_piBR_GradeSchoolSystem_picBR_GradeSchoolSystem_piBR_GradeSchoolSystem_picBR_GradeSchoolSystem_piGR_mainScanningPartDivisionof...
RT-PCR analysis was performed with a CFX real-time PCR instrument (Bio-Rad) and SYBR Green mixture (Roche). The relative expression of the genes was quantified with the 2−ΔΔCT calculation, using UBIQUITIN as the reference housekeeping gene for the expression analyses. The enrichment of ...