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For cell culture experiments, isolated microglia were resuspended and plated at a density of 106 cells/6 cm dish in microglia culturing medium containing DMEM/F12 (Gibco) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich) and 1% PenStrep (Gibco). For adult microglia, medium was ...
Cells were cultured in Dulbecco’s modified Eagle’s medium/F12 containing with 10% calf serum and 1% Pen/Strep. All cells were maintained at 37 °C with 5% CO2. Transfection Sequences used were as follows: FUT4 siRNA: 5′-GUUUGGAUGAACUUCGAGUTT-3′, 5′-ACUCGAAGUUCAUCCAAACTT-3′;...
Animals were then transferred into 250 mL of S-Medium supplemented with 1% Penn/Strep (Life Technologies), 0.1% nystatin (Sigma) and OP50 bacteria obtained from the growth of a 400 mL culture. Animals were grown at 20°C at low shaking for 96 hours and were harvested and cleaned using ...
Moreover, β-secretase 1 (BACE1) stability, β- and γ-secretase activity, as well as intracellular compartmentalization of PS1 and BACE1, but not of amyloid precursor protein (APP), nicastrin (Nct), presenilin enhancer 2 (Pen-2), or synaptophysin (Syp) were altered in the absence of Syt...
The 5-I-uridine was five times more effective than 5-Br-uridine for both enzymes. The ratios of IC50 values for PARP-1:PARP-2 are also approximately 1:1. The 5-Cl-uridine had a little effect on the activity of PARP-1, while PARP-2 was inhibited with an IC50 value of 196 μM. ...
Pen-Strep solutionEZSolution Penicillin/Streptomycin, Sterile-Filtered (100X) D-3-Deoxy-2-O-methyl-myo-inositol 1-[(R)-2-methoxy-3-(octadecyloxy)propyl hydrogen phosphateSH-5 (±)8,9-DHET (250 ug)(±)8,9-dihydroxy-5Z,11Z,14Z-eicosatrienoic acid; (±)8,9-DiHETrE; (±)8,9-DHET ...
A single clone was isolated and maintained in 10% FBS, DMEM, NEAA, Pen/Strep, and 500 pg/ml G418 and 100 pg/ml hygromycin B. [00123] To determine potency of inhibition, antibodies (100 nM to 0.002 nM) were incubated (1 hr; 25'C) with either 10 nM human big-ET-1 (SEQ ID NO...
πενθέω (pentheō), be sad, grieve, mourn (G4291); πένθος (penthos), grief, sadness,... G4344. περιπατέω, peripateō, go about, walk Collapse G4344 περιπατέω περιπατέω (peripateō), go about, walk (G4344); ἐμπερ...