Jurkat cells were seeded at a density of 1 × 106/mL in fresh cell culture medium and labeled with Calcein AM at 37 °C for 2 h. Labeling was monitored under a fluorescence microscope. After thoroughly washing to remove unincorporated dye, cells were resuspended into fresh culture m...
The experiments revealed that CH-4 inhibited the interaction of soluble form PD-L1 (sPD-L1) with PD-1 surface protein expressed by KG-1 cells. Investigations into CH-4 analogs revealed that CH-4.7 effectively blocked the PD-1/sPD-L1 interaction, but sustained the secretion of interleukin-2 ...
When colonies reached 80% confluence, cells were harvested with trypsin/EDTA and resuspended at 1 × 106 cells/T150 flask in ISM. Medium was changed every 3–4 days when cells reached 80–90% confluency, at which time cells were either frozen or passaged. MSC isolates were routinely ...
Cells were collected at 1-hour intervals, and cell density determined using a hemocytometer. For spotting assays, log phase cells grown in liquid YPAD were diluted to 1.0 × 106 cells/ml (~0.1 OD units) and 5-fold serial dilutions spotted (5 μl/spot) to YPAD or YPAG plates and ...
The proviral copies in these cells have been established at different times, and this allows the mixing of historical and more recent virus sequences. In conclusion, we have developed a new HIV-1 latency model system that uses the dox inducible HIV-rtTA virus variant. In combination with ...
MLE cells were seeded (1.5 × 106 cells/dish), and incubated with 800 nM of RSL3 in the presence or absence of FER-1 (0.4 μM) for 6 h. For Fe/Ascorbate experiment, MLE cells were treated with Fe2SO4 (10 μM) plus ascorbic acid (500 μM) for 45 min in the presence or ...
On Day 0, female BALB/c mice (n = 5 per group) were injected with 1.5 × 105 CIN.23 cells, IV. On Day 3, tumor-bearing mice were injected, IV, with 1.0 × 106 cells (as detailed in the figure) isolated from LV305- or mock-immunized donor mice. On Day 18, tumor ...
at a concentration of 1.0 × 106 cells/ml. Cell cultures were challenged for 2 h or 4 h at a cell to spore multiplicity of infection (MOI) of either 1:1 or 1:10 for microarray experiments. The term MOI used here simply refers to the number of spores added per cell. Each timepoint...
The short isoform of the murine Pim1 protein cap away was expressed in bacterial cells from the pGEX-2T-Pim1 vector as previously described [34]. The human PIM1 protein was expressed in bacterial cells from the pGEX-6P-1-PIM1 vector or in mammalian cells from the pcDNA™3.1/PIM1-V5-...
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