Itaconate is produced from the mitochondrial TCA cycle enzyme aconitase decarboxylase (encoded by immune responsive gene1; Irg1) that exerts immunomodulatory function in myeloid cells. However, the role of the Irg1/itaconate pathway in dendritic cells (D
we further explored whether they also exerted the tumor-promoting role through influencing CD8+T cell infiltration. Cd11b+Gr1+MDSCs isolated from MC38-derived tumors inId1f/fandId1Lyz-KOmice were cocultured with CD8+T cells. We found that depletion ofId1in ...
OVA-B16 TRCs were unable to affect expression of PD-1, IFN-γ, TNF-α, or CD107a in Ahr−/− OT-1 T cells (Figures 4F and S4C). Moreover, when we ectopically expressed AhR in HEK293T cells expressing a PD-1 luciferase reporter, we found that addition of Kyn resulted in a ...
3b). Besides, ICAM1 overexpression significantly improved the activated state of OT-I CTLs (CD69) in Lkb1-deficient tumors (Fig. 3b). Taken together, these data suggest that ICAM1 may be prerequisite for the homing of activated tumor-specific T cells in LKB1-deficient tumors. We also ...
1e). Results from flow cytometry analysis suggested that CCL7IRES−ZsGreen was expressed in CD11c+CD11b−H-2Kb−CD103− alveolar macrophages (AMs) but not in CD11c+CD11b−H-2Kb+CD103+ dendritic cells (DCs), CD11b+CD11c− cells, CD19+ B cells, CD4+ or CD8+ T cells (Fig...
The TT-EVs contained EVs from tumor and non-tumor cells. To identify whether tumor cells are the primary source of PD-L1+ TT-EVs, we analyzed the percentage of non-tumor cell-derived EVs in PD-L1+ TT-EVs. When labeled with CD45, CD31, CD41, or CD235a, markers corresponding to immu...
已知:H2SO3 Ka1=1.54×10-2Ka2=1.02×10-7HClO Ka=2.95×10-8H2CO3 Ka1=4.3×10-7Ka2=5.6×10-11Na2SO3溶液显碱性,其主要原因用离子方程式表示是___;少量二氧化碳通入NaClO溶液反应的离子方
Cells were washed and resuspended in 0.2 mL of PBS supplemented with 2% FBS, incubated with murine and human Fc Block (Becton Dickinson (BD), Franklin Lakes, NJ, USA) for 10 min at room temperature (RT), and then stained with antibodies specific to murine CD45 (30-F11; BioLegend, San...
Keywords: cardiac stem cells; immortalization; secretome; MCP-1; cardiomyocyte survival 1. Introduction Cardiac Sca-1+ cells showed cardiac stem cell (CSC) properties differentiating into cardiac and endothelial cells, as evidenced by in vitro and in vivo studies [1–5]. Knockdown of Sca-1 ...
Quebec is in some ways sui generis, but that does not mean there are not lessons applicable to other times and places. As longtime readers know, I spend part of the summer at the family cottage in Quebec and have over the years read a great deal of literature, and talked with numerous...