HSA-hG-CSF融合蛋白毕氏酵母融合表达为了延长G-CSF半衰期,利用甲醇酵母表达重组人血清白蛋白融合的集落细胞刺激因子(rHSA-G-CSF)。用PCR方法从人胎肝cDNA文库扩增出HSAcDNA序列,hG-CSFcDNA序列从大肠表达载体中酶切获取。将HSA和hG-CSF两片段连接后,克隆到酵母分泌型表达载体pGENYK中,酶切线性化后原生质体转化导入...
重组HSA-hG-CSF融合蛋白在毕赤酵母中的表达 为了延长G-CSF半衰期,利用甲醇酵母表达重组人血清白蛋白融合的集落细胞刺激因子(rHSA-G-CSF)。用PCR方法从人胎肝cDNA文库扩增出HSA cDNA序列,hG—CSFcDNA序列从大肠表... 王昌梅,王同映,杨志愉,... - 《中国生物工程杂志》 被引量: 29发表: 2006年 重组HSA-hG-...
HSA-hG-CSF融合蛋白毕氏酵母融合表达The complete gene of HSA-G-CSF was obtained by follow protocol: the HSA gene was obtained from the library of cDNA by PCR, and the gene of G-CSF was obtained from vector we constructed before. After ligation of the two genes, the complete gene was ...