V-ATP酶亚基克隆qRT-PCR【目的】克隆获得草地贪夜蛾Spodoptera frugiperda V-ATP酶(Vacuolar-type proton,ATPase)V1结构域A,B,C和D亚基的cDNA序列,分析其在草地贪夜蛾不同生长阶段的表达情况,为筛选新型农药的靶点奠定基础.【方法】利用PCR技术获得草地贪夜蛾V-ATP酶V1结构域A,B,C和D亚基基因的ORF区域,采用qRT...
[Objectives] To clone the cDNA sequences of the A, B, C and D subunit genes of the Spodoptera frugiperda V-ATPase V1 region and compare the expression of these genes in different developmental stages in order to both improve the prevention and control of S. ...
克隆荧光定量PCR[目的]液泡型ATP酶(vacuolar-type proton ATPase,V-ATP酶)维持着适合真核细胞内膜室生化功能的pH环境.本研究旨在克隆草地贪夜蛾Spodoptera frugiperda V-ATP酶V1结构域E,F,G和H亚基基因,并对其时空表达谱进行分析.[方法]采用RT-PCR技术克隆了草地贪夜蛾V-ATP酶E,F,G和H亚基基因,通过实时荧光...