摘要 一种大黄鱼头肾巨噬细胞体外模型的构建技术,其特征是利用31%/45%梯度的Percoll水平离心分离鱼类头肾巨噬细胞,并在22℃条件下培养在每升含有50ml胎牛血清、10万UI青链霉素、5万UI肝素、10mmol4-羟乙基哌嗪乙磺酸(HEPES)和0.5g葡萄糖,pH为7.4的L15复合培养基中。本发明具有如下优点:(1)31%/45%的Percoll...
大黄鱼头肾巨噬细胞体外模型的构建 development of a short-term in vitro model of macrophages from large yellow croaker, larmichthys crocea (richardson, 1846).pdf 2015-01-28上传 大黄鱼头肾巨噬细胞体外模型的构建 development of a short-term in vitro model of macrophages from large yellow croaker,...
(DHA, 22:6n-3)对离体大黄鱼头肾巨噬细胞营养生理及免疫力的影响.主要研究结果如下: (1)通过优化Percoll密度梯度离心技术,从大黄鱼头肾组织中分离纯化巨噬细胞,并优化细胞培养条件.对所分离细胞进行形态学分析,普通光镜和电镜超微结构观察以及细胞功能验证实验.实验结果表明:相比34%/51% Percoll梯度分离液,31%/...
大黄鱼头肾巨噬细胞体外模型的构建 development of a short-term in vitro model of macrophages from large yellow croaker, larmichthys crocea (richardson, 1846).pdf 2015-01-04上传 大黄鱼头肾巨噬细胞体外模型的构建 development of a short-term in vitro model of macrophages from large yellow croaker,...