实验建议PurKine™ Anti-Flag树脂(4%交联)是用于纯化含有 FLAG-tag 的融合蛋白的通用性亲和产品,可以重复使用而无需再生,但随着非特异性结合的蛋白的增多和蛋白的聚集,往往造成流速和结合载量都下降,这时可以对树脂进行清洗,恢复性能。样品在上样前建议离心或用 0.22μm 或 0.45μm 滤膜过滤,减少杂质,提高蛋白...
Anti-DYKDDDDKAffinity Beads是以抗flag(DYKDDDDK)抗体为亲和配体,一步纯化原核、酵母或哺乳动物细胞表达的flag标签融合蛋白。Anti-DYKDDDDK Affinity Beads以4%琼脂糖凝胶为基质,杂蛋白非特异性结合少,可用于flag标签融合蛋白的纯化和免疫沉淀(IP)。 表1.Anti-DYKDDDDKAffinity Beads产品性能...
目录价2600 15013182710(微信) 产品详情产品评论(0) Flag标签是一个由八个亲水氨基酸组成的多肽片段,定位在融合蛋白表面,因此更易与抗体结合以及被肠激酶分解。Anti-DYKDDDDK S1 Affinity Beads是以优化改造后的抗Flag(DYKDDDDK)抗体为亲和配体,一步纯化原核、酵母或哺乳动物细胞表达的Flag标签融合蛋白。Anti-DYKDDDDK ...
背景The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It ...
别名DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope 图片及说明 Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag 兔 多克隆 antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.
别名DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope 图片及说明 Fig. Anti-DDDDK Tag Mouse Monoclonal Antibody, Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant DDDDK tagged proteins.
别名DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope 图片及说明 Fig. Anti-DDDDK Tag Mouse Monoclonal Antibody, Agarose are convenient for the immunoprecipitation (IP) of recombinant DDDDK tagged proteins.
别名DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope 图片及说明 Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI. Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransf...
背景The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It ...