The invention discloses a genetic engineering preparation method for mu-conotoxin-KIIIA, which comprises: inserting a mu-conotoxin-KIIIA gene into a prokaryotic expression vector with Trx-tag to construct a recombinant prokaryotic expression vector; and transferring Escherichia coli with the recombinant ...
Folding similarity of the outer pore region in prokaryotic and eukaryotic sodium channels revealed by docking of conotoxins GIIIA, PIIIA, and KIIIA in a Na... Voltage-gated sodium channels are targets for many Zhorov,Boris,S.,... - 《Journal of General Physiology》 被引量: 33发表: 2014...
For KIIIA, we have identified a significant toxin binding "microsite" near the outer ring charge in channel domain III. This site is an important determinant of the targeting of the toxin to inhibit human Nav1.7, a channel important in pain signalling. Neutral substitut...