Then the samples were diluted with cold reaction buffer (PBS with 5% BSA and 0.03% Tween-20, supplemented with protease inhibitor cocktail), and centrifuged at 16,000 g for 20 min at 4 °C. The Aβ in the total brain was analyzed with human Aβ42 (KHB3441, Invitrogen), human...
The kinetic analysis showed a bi-phasic inhibition mode of compound 11, that is a fast-loose slow–tight interaction with d-secretase. Such bi-phasic inhibition can be rationalized by conformational rearrangements within the protein–inhibitor complex and are often observed with allosteric inhibition30...
Briefly, 150 mg frontal cortex tissue was minced with a scalpel blade in 1 ml of homogenization buffer (0.25 M sucrose, 1 mM MgCl2, 10 mM Tris-HCl, pH 7.4, supplemented with protease inhibitor NATURE COMMUNICATIONS | 6:8762 | DOI: 10.1038/ncomms9762 | www.nature.com/naturecommunications ...
we reported that AEP cleaves SET that acts as a DNase inhibitor and inactivates SET, leading to DNA damage and neuronal cell death in a model of stroke5. In addition, SET also functions as a PP2A inhibitor. AEP-cleaved SET blocks the phosphatase activity of PP2A and results in Tau hyperph...