Then the samples were diluted with cold reaction buffer (PBS with 5% BSA and 0.03% Tween-20, supplemented with protease inhibitor cocktail), and centrifuged at 16,000 g for 20 min at 4 °C. The Aβ in the total brain was analyzed with human Aβ42 (KHB3441, Invitrogen), human...
We performed a high-throughput screen and identified a non-toxic and selective d-secretase inhibitor, termed compound 11, that specifically blocks d-secretase but not other related cysteine proteases. Co-crystal structure analysis revealed a dual active site-directed and allosteric inhibition mode of ...
Cystatin C interacts with active AEP in the lysosome and acts as an endogenous inhibitor of AEP26,27. Together, these observations led us to hypothesize that AEP plays an important role in AD pathogenesis. In this report, we show that APP is a physiological substrate of AEP. AEP increases ...
we reported that AEP cleaves SET that acts as a DNase inhibitor and inactivates SET, leading to DNA damage and neuronal cell death in a model of stroke5. In addition, SET also functions as a PP2A inhibitor. AEP-cleaved SET blocks the phosphatase activity of PP2A and results in Tau hyperph...