Create constitutively GTP bound GTPases Activate small G-proteins Material Guanosine gamma thio-phosphate (GTPγS), a non-hydrolyzable GTP analog. Upon resuspension with 50 µl de-ionized water you will have a 20 mM stock which is enough for 50 assays of small G-protein activation. ...
Addition of guanosine 5'-(3-O-thio)triphosphate (GTP gamma S) to the membrane fraction isolated from rabbit peritoneal neutrophils results in the solubilization of several proteins from the membrane. The major proteins are of 180 kDa (myosin) and 43 kDa (actin). The effect is observed with ...
GT Babkina,J Jonák,I Rychlík 摘要: New types of azidoaryl analogs of GTP: γ-(4-azido)anilide of GTP (I), γ-( N-(4-azidobenzyl)- N-methyl)amide of GTP (II) and of GDP: β-(4-azido)anilide of GDP (III), β-( N-(4-azidobenzyl)- N-methyl)amide of GDP (IV) have ...
The small G protein Rap2A has been crystallized in complex with GDP, GTP and GTPS complex. Tyr32 is conserved in many small G proteins, which probably also form this hydrogen bond with GTP. In addition, Tyr32 is structurally equivalent to a conserved arginine that binds GTP in trimeric G...
The small G protein Rap2A has been crystallized in complex with GDP, GTP and GTPS complex. Tyr32 is conserved in many small G proteins, which probably also... J.,Cherfils - 《Embo Journal》 被引量: 173发表: 1997年 Structure of the small G protein Rap2 in a non-catalytic complex ...