Methods: APP Tg mice (Tg2576) which are usually maintained by B6/SJL hybrid mice were crossed onto C57BL6/J, SJL/J and DBA2/J inbred strains.The levels of Abeta40 and Abeta42 in Triton and GuHCl fraction from cortex were measured by ELISA. The mRNA level of APP was measured in ...
Methods The protofibril-selective monoclonal antibody mAb158 (IgG2a) was given by weekly intraperitoneal injections to aged tg-APP ArcSwe mice, and clearance of soluble brain protofibrils was evaluated in brain TBS extract using a mAb158-based sandwich ELISA, as well as in the CSF using a 82...
Furthermore, reduced levels of insoluble A beta(1-40) (69%) and A beta(1-42) (50%) after icariin treatment were determined in the brain by enzyme-linked immunosorbent assay (ELISA). Western blot analysis indicated the downregulation of APP expression after icariin treatment, and double ...
4.9. SAA ELISAs on Serum and Brain SAA was quantified from serum and brain extracts using the Mouse Serum Amyloid A DuoSet ELISA (R and D Systems, Minneapolis, MN, USA) according to kit instructions. We have validated these kits for SAA measurement in brain and blood using tissue from mic...
28 conjugate were analyzed using a standard ELISA. A??42 and A??40 amyloid burden, cerebral amyloid angiopathy (CAA), astrocytosis, and microgliosis in the brain of immunized and control mice were detected using immunohistochemistry. Additionally, cored plaques and cerebral vascular micro...
However, as the ELISA is specific for human pE3-42Ab we were unable to address if endogenous rodent pE3-42Ab could be present. Consequently, a monoclonal antibody against mouse and rat pE3Ab was subsequently generated and data from the characterisation and tissue analysis is presented. ...
Plasma was collected every week after the administration and anti-Aβ antibody levels were measured by ELISA. A passive avoidance test was performed one week after the final treatment. Two weeks after the final treatment, the levels of Aβ in brain, CSF and plasma were measured by ELISA. ...
Soluble Aβ x-40 and Aβ x-42 concentrations were determined by sandwich ELISA following TBS extraction from sonicated hemibrains. Citrate synthase activity in soleus muscle was determined to assess muscle oxidative capacity, an indicator of effectiveness of an exercise training program in improving ...
The levels of different Ab species and other APP processing fragments were analyzed using commercially available ELISA (Invitrogen & Innogenetics) or MSD technologies. Extensive statistical analysis was performed on all data. Results We found that the levels of both endogenous mouse and transgenic human...
In vitro cell biological study, immunohistological and ELISA (enzyme-linked immunosorbent assay) analyses were used to assess its neuroprotective effects against Aβ-induced cell death, and the Aβ accumulation and plaque deposition in the brain. In vitro study with SH-SY5Y neuroblastoma cells ...