ELISA results using whole brain homogenates indicated that norclozapine (8 mg/kg) significantly decreased Aβ protein levels whereas scopolamine (2 mg/kg) and tolterodine (2 mg/kg) had no significant effect on Aβ levels. Analysis of ChAT proteins in brain homogenates of treated 3xTg-AD mice...
Sandwich ELISA analysis of extractable Abeta in the brain of treated indicates that both Abeta40 and the longer, more amyloidogenic form of the , Abeta42, were significantly reduced. These data provide the first direct evidence that compounds identified by their ability to reduce Abeta ...
Furthermore, reduced levels of insoluble A beta(1-40) (69%) and A beta(1-42) (50%) after icariin treatment were determined in the brain by enzyme-linked immunosorbent assay (ELISA). Western blot analysis indicated the downregulation of APP expression after icariin treatment, and double ...
Methods The protofibril-selective monoclonal antibody mAb158 (IgG2a) was given by weekly intraperitoneal injections to aged tg-APP ArcSwe mice, and clearance of soluble brain protofibrils was evaluated in brain TBS extract using a mAb158-based sandwich ELISA, as well as in the CSF using a 82...
2.4. Amyloid-β ELISA Frozen brain cortical hemispheres from male and female 3XTg-AD mice were used to isolate diethylamine (DEA; Sigma-Aldrich) soluble and formic acid (FA; Sigma-Aldrich) soluble Aβ applying the method described by (Casali and Landreth, 2016). In brief, mechanically homogen...
Plasma was collected every week after the administration and anti-Aβ antibody levels were measured by ELISA. A passive avoidance test was performed one week after the final treatment. Two weeks after the final treatment, the levels of Aβ in brain, CSF and plasma were measured by ELISA. ...
Soluble Aβ x-40 and Aβ x-42 concentrations were determined by sandwich ELISA following TBS extraction from sonicated hemibrains. Citrate synthase activity in soleus muscle was determined to assess muscle oxidative capacity, an indicator of effectiveness of an exercise training program in improving ...
However, as the ELISA is specific for human pE3-42Ab we were unable to address if endogenous rodent pE3-42Ab could be present. Consequently, a monoclonal antibody against mouse and rat pE3Ab was subsequently generated and data from the characterisation and tissue analysis is presented. ...
The levels of different Ab species and other APP processing fragments were analyzed using commercially available ELISA (Invitrogen & Innogenetics) or MSD technologies. Extensive statistical analysis was performed on all data. Results We found that the levels of both endogenous mouse and transgenic human...
In vitro cell biological study, immunohistological and ELISA (enzyme-linked immunosorbent assay) analyses were used to assess its neuroprotective effects against Aβ-induced cell death, and the Aβ accumulation and plaque deposition in the brain. In vitro study with SH-SY5Y neuroblastoma cells ...