Furthermore, analysis of only a few hundred cells per sample is possible. We examined the sensitivity of this method by comparing the percentages of positive cells in a spleen cell suspension after staining with a panel of monoclonal antibodies followed by analysis with the present immuno-beta-...
Apoptotic activity of AuNPs was determined against HeLa cells using annexin V/ propidium iodide double staining by flow cytometric analysis. The AuNPs exhibited excellent efficacy against these cell lines and future prospects of usage as potential nano-drugs and drug delivery vehicles....
This report shows: (1) a therapeutic gene can be linked to a marker gene by an IRES element achieving equivalent expression of both proteins; (2) the co-expression of a marker gene makes fluorescein-di-beta-D-galactopyranoside staining possible, and consequently separation of cells expressing ...
Azo dye methods involving naphthyl substrates showed high and nonspecific background staining to the exine. The indigogenic method, employing 5-bromo-4-chloro-3-indoxyl 尾- d -galactoside (X-gal) as the enzyme substrate, gave specific opaque-blue final reaction pproduct, while mutant pollen ...
Enhancer trap fly strains show cell- and/or tissue-specific 尾-galactosidase expression which can be visualized by a simple X-gal staining procedure. To combine the power of the enhancer trap approach with electron microscopy, we have improved the fixation and staining conditions, which allow ...
(H) Quantitative analysis of the relationship between the width of the band of β-gal-pH6 staining and the thickness of the olfactory epithelium (OEw) during postnatal mouse development. Data are expressed as mean ± SEM. Statistical significance is indicated by asterisks (**p< 0.01). The ...
Figure 9.Reduced microgliosis in mβ-Gal:RTB treatedβ-Gal−/−mice. IF staining of microglia in the thalamic region of WT,β-Gal−/−and mβ-Gal:RTB injectedβ-Gal−/−mice using anti-Iba1 antibody. Scale bar: 100 µm. ...
Detailed procedures used in FCM (cell preparation and DNA staining; flow cytometry analysis) and PFGE (immobilization of yeast DNA in agarose plugs; pulsed-field gel electrophoresis) have been described previously by Białkowska et al. [48]. 4.3. Biochemical and Physiological Characterization of ...