coli. Phylotyping was done using Clermont phylo-typing method, targeting arpA, ChuA, and YjaA genes, along with the DNA fragment TspE4.C2. Convetional PCR method was used to confirm the presence of bla genes among 39 phenotypically confirmed ESBL producing E. coli. The results showed ...
Out of the 10 β-lactamase genes, four belonged to ESBL (,, CTX, and); three to MBL (-1,, and); and three to ABL (,and) class of genes. The different categories of DEC were estimated for β-lactamases production using a set of conventional phenotypic tests, followed by detection ...
Backbone genes for replication, conjugal transfer, and plasmid maintenance are colored red, and other are highlighted in green, brown, and light blue, respectively. resistance genes are in fuchsia. Insertion elements, integrase bgleanCeAsE,-1ananddgebnlaeAsFfMor heavy metal resistance (ars and mer...
Multi- and pan-antibiotic-resistant bacteria carry many resistance genetic determinants, including several types of β-lactamase genes [33], which explain the need to develop multiplex methods to determine them. For this work, we have selected four relevant β-lactamases genes: TEM-1, CTX-M-3,...
Two of the isolates had only bla SHV gene, whereas 1 isolate had a combination of bla TEM and bla SHV genes. Three of these were resistant to all the drugs tested, while two were sensitive to getamicin and one to ciprofloxacin. None of the E. coli strains possessed bla CTXM-2 , ...
Hybridization studies with cloned aeromonas beta-lactamase genes indicated that the cephalosporinase gene, cepS, is almost ubiquitous for the three species tested. The cphA gene cross-hybridized with all isolates of A. veronii and A. hydrophila but not to A. caviae isolates. In contrast, ...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook beta-lactamase (redirected fromBetalactamase) Thesaurus Medical Encyclopedia Related to Betalactamase:ESBL be·ta-lac·ta·mase (bā′tə-lăk′tə-mās′, -māz′, bē′-) ...
The corresponding structural genes were amplified by the polymerase chain reaction. Nucleotide sequences were determined by direct sequencing of the amplified products. Analysis of the nucleotide and the deduced amino acid sequences showed that CAZ-2, CAZ-6, and CAZ-7 are derived from TEM-2 by ...
The genes blaCTX-M-1 and qnrS1 were detected on highly related IncI1 plasmids assigned to clonal complex 3 (ST3, ST38) and on non-related IncN plasmids of ST1 and ST3, respectively. The gene qnrS1 was located on related IncX1 plasmids. Dissemination of antibiotic resistance is associated...
ESBL-producing bacteria were identified by the double disk method in line with CLSI guidelines, and the most common ESBL genes were identified by PCR assay in phenotypical ESBL positive strains. Results Data derived from a total of 4083 consecutive clinical samples were included in this study. ...