The present invention relates to the use of a nucleic acid fragment or part thereof encoding β-ketoacyl ACP synthase II (KASII), particularly which derived from to enhance the production and the accumulation of very long chain fatty acids (VLCFA) in a plant, oil crops of particular.UMI ...
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With the aim of elucidating the mechanisms involved in the biosynthesis of medium-chain fatty acids in Cuphea lanceolata Ait., a crop accumulating up to 90% decanoic acid in seed triacylglycerols, cDNA clones of a β-ketoacyl-acyl carrier protein (ACP) synthase IV (clKAS IV, EC 2.3.1.41)...
Cvc- mutants of Escherichia coli are deficient in the synthesis of cis-vaccenic acid and in the temperature control of fatty acid synthesis. In this communication, it is demonstrated that these mutants lack beta-ketoacyl-acyl carrier protein synthase II. The deficiencies in cis-vaccenate synthesis...
beta-Ketoacyl-[acyl carrier protein (ACP)] synthase forms new carbon-carbon bonds in three steps: transfer of an acyl primer from ACP to the enzyme, decarboxylation of the elongating substrate and its condensation with the acyl primer substrate. Six residues of Escherichia coli beta-ketoacyl-ACP...
Fatty Acid Biosynthesis: Fatty Acid Synthase. Molecular aspects of β-ketoacyl synthase (KAS) catalysis. Structure-function, Fedtsyre, Proteinkemi, Molekylærbiologi PV Wettsteinknowles,JG Olsen,S Larsen 被引量: 6发表: 2001年 Molecular aspects of \\u03b2-ketoacyl synthase (KAS) catalysis Str...
The crystal structure of beta-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54 A resolution and its relationship to other condensin... Moche M, Dehesh K, Edwards P, Lindqvist Y, The crystal structure of beta-ketoacyl-acyl carrier protein synthase II from Synechocystis ...
beta-Ketoacyl-acyl carrier protein synthase III (FabH) is a condensing enzyme that plays central roles in fatty acid biosynthesis. Three-dimensional structures of E. coli FabH in the presence and absence of ligands have been refined to 1.46 ANG resolution. The structures of improved accuracy ...
coli gene ( fabH ) encoding β-ketoacyl-carrier protein synthase III. In addition, a leucine tRNA gene ( trnL (GAG)) was detected just downstream. Neither of these genes are encoded on the chloroplast genomes of land plants.关键词: chloroplast genome fabH Porphyra umbilicalis trnL(GAG ...
To test this hypothesis, we expressed the AT and KS domains of the mycocerosic acid synthase (MAS) gene from Mycobacterium bovis BCG in Escherichia coli and examined whether they confer to synthases that normally do not use methylmalonyl-CoA the ability to incorporate methylmalonyl-CoA into fatty...