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As expected from HEX enzymatic function requirements, overexpression in vivo of HEXA and B combined, but not either of the subunits expressed alone, increased HEX activity as evidenced by histochemical assays. Biochemically, such HEX gene expression resulted in increased conversion of GM2 to its ...
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexosaminidase B Beta (HEXb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% ...
Bacterial contamination positively interferes with beta-glucosidase and hexosaminidase assays in white blood cell lysatesbacterial contaminationbeta-glucosidasewhite blood cells.Deficiency of lysosomal enzymes in lipid storage disorders relies on assay of most of these enzymes in white blood cell (WBC) ...
Identifies an active acidic residue in the catalytic site of the lysosomal glycosidase beta-hexosaminidase. Protein homology alignments; Site-directed mutagenesis and vector constructions; Cell culture; Permanent DNA transfection; Hexosaminidase enzyme assays.TseRoderickVavougiosGeorge...