The beta-agarase is used for degrading agarose to produce neoagarotetraose and can specifically degrade agarose to produce the unique product, namely, neoagarotetraose; and therefore, the beta-agarase can be used for preparing high-purity neoagarotetraose; the conversion rate of the beta-agarase ...
The invention provides beta-agarase. An amino acid sequence of the beta-agarase is SEQ (Sequence) ID (Identity) NO.(Number) 1; genes of the beta-agarase are coded; one of nucleotide sequences of the beta-agarase is SEQ ID NO. 2. According to the beta-agarase, agar can be ...
The present invention relates to beta-agarase derived from Alteromonas sp. GNUM-1 and, specifically, to beta-agarase having an amino acid sequence of SEQ ID NO: 1 derived from Alteromonas sp. GNUM-1, a gene thereof, a beta-agarase recombinant expression vector comprising the gene DNA, a ...
Novel two types of beta -agarases derived from a marine bacterium screened and isolated from marine sediment, include beta -agarase A of a molecular weight of about 90,000 and beta -agarase B of a molecular weight of about 98,000. beta -agarase A has an enzyme activity of hydrolyzing ...
Purification and characterization of a novel beta- agarase from Vibrio sp AP-2 [J].Europe Journal of Biochemistry,1990,187(2):461-465.Aoki T., Araki T., Kitamikado M. Purification and characterization of a novel beta-agarase from Vibrio sp. AP-2. Eur. J. Biochem. 1990; 187 :461–...
Expression of beta -agarase I DagA in prokaryotic cell and its activity identificationYan-ShengZ.Bao-LiW.DongQ.CHINESE JOURNAL OF AGRICULTURAL BIOTECHNOLOGY
Parallel substrate binding sites in aβ-agarase suggest a novel mode of action on double-helical agarose. Allouch, J,Helbert, W,Henrissat, B,Czjzek, M. Structure . 2004Allouch J, Helbert W, Henrissat B, Czjzek M. Parallel substrate binding sites in a β-agarase suggest a novel ...
Characterization of a novel β-agarase from marine Alteromonas sp. SY37-12 and its degrading products. JX Wang,HJ Mou,XL Jiang,HS Guan. Applied Microbiology and Biotechnology . 2006Wang JX, Mou HJ, Jiang XL, Guan HS (2006) Characterization of a novel beta-agarase from marine Alteromonas sp...
Since the strain A3(2)_M22-2C43 according to the present invention expresses a DagB mutant enzyme expressing little or no DagB beta-agarase or exhibiting little or no beta-agarase activity, there is no need for separate isolation and purification of DagA enzymes from culture fluid, and the ...
The invention aims to provide a beta-agarase and application thereof. The beta-agarase is one capable of degrading agarose to produce single Neoagarobiose, and the amino acid sequence is shown in SEQ ID NO:1. According to the invention, the beta-agarase can degrade agarose to produce single...