Wnt蛋白与细胞表面的Frz、LRP5/6结合形成三聚体,将信号传递并活化胞质内蓬乱蛋白(dishevelled,Dsh或Dvl),减弱由β-catenin与轴蛋白(axin)、糖原合成酶激酶-3β(glycogen synthase kinase-3β,GSK-3β)、结直肠腺瘤性息肉基因(adenomatous polyposis,APC)组成...
▐Wnt 通路的激活途径Wnt 的激活是通过不同的 Wnt-蛋白质配体结合到 Frizzled 家族细胞表面受体,从而传递生物学信号至细胞内,包括三种途径[8]:(1) 经典 Wnt/β-catenin 途径:Wnt 基因在肿瘤细胞中被异常激活,磷酸化激活胞质内的 Dsh 蛋白,抑制 GSK3β/APC/Axin 复合物中的关键成分 GSK3β 活性,阻止 GSK3...
然而在多种癌症细胞内发现这些 Wnt/β-catenin 通路负调控因子的基因发生突变和缺失,β-catenin 在细胞质内大量聚集随后转移至核内与 T 细胞因子/淋巴增强因子(Tcellfactor/Lymphoid enhancing factor, TCF/LEF)等转录因子形成复合物,激活下游...
6月,山东大学研究团队发现Wnt/β-catenin信号通过靶向GPX4抑制胃癌细胞铁死亡发生,并在“Cell Death & Differentiation”(2022 IF:12.067)在线发表了题为“Wnt/beta-catenin signaling confers ferroptosis resistance by targeting GPX4 in gastric cancer”的研究结果 10月,中山大学易陈菊、牛建钦团队发现激活Wnt/β-cate...
Wnt/β-catenin signaling: components, mechanisms, and diseases 在wnt/beta-catenin通路的研究中,抗体是十分重要的研究工具。就如同在这篇文献中提到的,许多科学家使用免疫荧光,免疫组化和ELISA等方法检测该信号通路中的关键分子,比如Axin,beta-catenin,GSK3等等,从而能够对各个分子进行定性和定量研究。因此,能否挑选...
WNT/β-catenin通路中的蛋白复合物异常相分离与癌症密切相关,为更好地理解蛋白复合物如何形成功能性凝聚体,以及小分子药物如何正确分布到凝聚体中,还需要更多相分离,蛋白质组学,AI辅助药物设计等相关研究。 3. 靶向β-catenin磷酸化位点和核转运 β-catenin被多亚基复合物磷酸化,不同的磷酸化位点在正常发育和疾病发...
3. MacDonald BT, et al. Wnt/beta-cateninsignaling: components, mechanisms, and diseases. Dev Cell. 2009Jul;17(1):9-26.4. T Zhan, etal. Wnt signaling in cancer. Oncogene. 2017 Mar;36(11):1461-1473.5. Theka I, et al. Wnt/β-catenin signaling pathway safeguards epigenetic stability ...
[11] Zhu D, et al. hsa-miR-199b-3p suppresses osteosarcoma progression by targeting CCDC88A, inhibiting epithelial-to-mesenchymal transition, and Wnt/beta-catenin signaling pathway. Sci Rep. 2023 Aug 2, 13(1): 12544. [12] Ling DJ, et al. MicroRNA-145 inhibits lung cancer cell metastasi...
group domain. They are involved in the Wnt signaling pathway, particularly during embryonic [2] and stem-cell development,[3] but also had been found to play a role in cancer[4] and diabetes.[5] TCF/LEF factors recruit the coactivator beta-catenin to enhancer elements of genes they target...
7. He, X.C., et al., BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-beta-catenin signaling. Nat Genet, 2004. 36(10): p. 1117-21.8. Qi, Z., et al., BMP restricts stemness of intestinal Lgr5+ stem cells by directly suppressing their signature ...