CRISPR/Cas9DNA end resectionGenome-wide screenHRNHEJRPADNA double-strand breaks (DSBs) are mainly repaired by homologous recombination (HR) and non-homologous end joining (NHEJ). The choice of HR or NHEJ is dictated in part by whether the broken DNA ends are resected to generate extended ...
A whole genome CRISPR-Cas9 screening pipeline was set up in the Cellular Generation and Phenotyping cell culture core facility at the Wellcome Sanger Institute. The pipeline consists of the following three stages: Successfully transduced cells were selected with puromycin on day 3 and expanded, mainta...
This genome-wide screening strategy, therefore, provides an invaluable approach to the study of neutrophils and provides a resource that will inform future studies of cell migration in these and other rapidly migrating cells.Similar content being viewed by others The neutrotime transcriptional signature...
Using the TALEN and CRISPR-Cas9 systems, Hotta and colleagues restored the disease-causing mutation of the dystrophin gene by exon skipping, frameshift, and exon knockin approaches in Duchenne muscular dystrophy patient-derived iPSCs. Ri... H Li,N Fujimoto,N Sasakawa,... - 《Stem Cell Reports...
The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include plant biotechnology and functional genomics research. However, the sp
(n = 75) in an independent HCC patient cohort. The relative intensity of FGA and p-TYK2 were normalized to β-actin. Source gels in Supplementary Fig.5.s, A proposed model illustrating the role of the FGA/TYK2/STAT3 axis during HCC tumorigenesis. Wildtype and mutated forms ofFGAwere...
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specific characteristics of the CRISPR-Cas system in AB43 are described as follows: (1) The strain AB43 carries a complete type I-Fb CRISPR-Cas system; (2) Homology analysis confirmed that thecasgenes in AB43 share high sequence similarity with the same subtypecasgenes; (3) A total of ...
Whole genome CRISPR/Cas9 screen identifies established and novel genes required for ovarian cancer disseminationdoi:10.1016/j.ygyno.2019.03.108L.J. WheelerZ.L. WatsonT.M. YamamotoK.D. SullivanB.G. BitlerGynecologic Oncology
et al. CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets. Nat. Methods 14, 607–614 (2017). CAS PubMed PubMed Central Google Scholar Koche, R. P. et al. Extrachromosomal circular DNA drives oncogenic genome remodeling in neuroblastoma. Nat....