To create the pGL4.10-hNR0B1 construct for dual-luciferase reporter assays, the human NR0B1 (hNR0B1) promoter was PCR amplified (for PCR primer sequences see Supplementary Table S5) and cloned into the pGL4.10 vector (Promega Corporation, Madison, WI, USA) using the XhoI and HindIII ...
along with 4.80 million insertions and deletions (INDELs ≤100 bp; 1.16 million/individual for domestic sheep versus 1.38 million/individual for Asiatic mouflon; Mann-Whitney,P < 0.001) (Supplementary Fig.1and Supplementary Data3) in the downstream analyses. In addition, the high-depth who...
All samples were blinded and phylogenetic trees were returned using the PHYLIP program Dnaml, Dnapars and Dnadist [27]. Short Tandem Repeat (STR) Genotyping All samples were genotyped using the PowerPlex® 16 System (Promega Corp, Madison, WI) on a 3130×L genetic analyzer with a 36 cm ...
The study was approved by the University of Wisconsin-Madison Institutional Review Board, and all methods were carried out in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All...
Ligated DNA was size-selected for lengths between 200 and 350 bp and subjected to exonic hybrid capture using SeqCap EZ Human Exome Library v3.0 (Roche Nimblegen, Madison, WI, USA). Each of the captured libraries were multiplexed and sequenced on multiple Illumina HiSeq 2000 (Illumina, San...
University of Wisconsin–Madison, Boulder Junction, WI, USA; 9Department of Geoscience, Madison, WI, USA; 10University of Wisconsin, Center for Limnology, Madison, WI, USA and 11Department of Bacteriology, University of Wisconsin–Madison, Madison, WI, USA Disturbances act as powerful structuring ...
To create the pGL4.10-hNR0B1 construct for dual-luciferase reporter assays, the human NR0B1 (hNR0B1) promoter was PCR amplified (for PCR primer sequences see Supplementary Table S5) and cloned into the pGL4.10 vector (Promega Corporation, Madison, WI, USA) using the XhoI and HindIII ...
DNA extraction and quantification Genomic DNA was extracted using an automated DNA/ RNA extraction system (Maxwell16, Promega, Madison, WI, USA). The quality, quantity and purity of the DNA were determined using an agarose gel NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA,...
All samples were blinded and phylogenetic trees were returned using the PHYLIP program Dnaml, Dnapars and Dnadist [27]. Short Tandem Repeat (STR) Genotyping All samples were genotyped using the PowerPlex® 16 System (Promega Corp, Madison, WI) on a 3130×L genetic analyzer with a 36 cm ...
De novo genome assembly was performed using Lasergene SeqMan NGen® and SeqMan Pro 12 (DNASTAR Inc., Madison, WI, USA). The length of the 43 WGSs generated ranged from 7347 bp to 7423 bp, including the ORF sequence encoding the viral polyprotein. To account for difficulties in resolving...