PTH was inversely correlated with serum 25(OH)D (r = −0.311, p < 0.001), but not with calcium intake or serum calcium (r = −0.087 and 0.057, respectively, both p > 0.05). Analysis of variance showed that while serum 25(OH)D (dichotomised at 50 nmol/L) had a significant ...
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Flow cytometry data was analysed by Mann-Whitney U test. An unpaired Student's t-test was used to compare cytokine levels between individuals with an insufficient (<50 nmol/L) and sufficient (>50 nmol/L) 25(OH)D status. Spearman's correlation test was performed for plasma 25(OH)D and ...
3. Results 3.1. Growth-Inhibitory Effects of MKH Derivatives on HL60 and HL-60R Cells To assess the growth inhibitory effects of MKH derivatives (MKH-DMG and MKH- SUC) on HL60 and HL-60R cell lines, a dose–cell viability plot at 72 h after treatment with the test drugs was ...
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Reagents and Cell Culture To begin, 1α,25-dihydroxyvitamin D3 (D1530, Sigma Aldrich, St Louis, MO, USA) was reconstituted in ethanol (10 µM stock solution). Human skin fibroblasts from the HVDRR patient with a homozygous p.Arg30* VDR mutation [21,22] and from an age/sex-matched...