HIV-1 Vif promotes the degradation of host anti-retroviral factor family, APOBEC3 proteins via the recruitment of a multi-subunit E3 ubiquitin ligase complex. The complex is composed of a scaffold protein, Cullin 5 (Cul5), RING-box protein (Rbx), a SOCS
The human APOBEC3G (A3G) protein activity is associated with innate immunity against HIV-1 by inducing high rates of guanosines to adenosines (G-to-A) mutations (viz., hypermutation) in the viral DNA. If hypermutation is not enough to disrupt the reading
3B). These results suggest that Vif and APOBEC3G interact directly and form a complex in vivo, which accelerates the degradation of APOBEC3G via the ubiquitin-proteasome pathway. Download: Download high-res image (147KB) Download: Download full-size image Fig. 3. Co-immunoprecipitation of Vif ...
from triplicate wells. full size image to determine whether elob knockdown would result in impairment of hiv-1 vif function, we co-transfected pnl4-3Δvif and vr-vif (without a tag) or empty plasmid vr1012 in the presence of sieb1 or sinc. in the sinc-transfected 293t cells, the ...
Primers that distinguished the full-length and exon V-deleted mRNA detected only the latter in all of the tissues (data not shown). This form was therefore used in the subsequent experiments. Taken together, the findings suggested that APOBEC3G is mainly expressed in lymphocytes and monocytes ...
This region has been predicted to form an alpha helix that fitted into the hydrophobic pocket of ElonginC. Vif is known to bind ElonginC through BC-box and Cullin5 through hydrophobic residues within a zinc binding region formed by a conserved HCCH domain34; this region was conserved in our...
(3) EloB-EloC but not CBF-β could greatly enhance the folding of full-length Vif in Escherichia coli. (4) The over-expression of EloB or the N-terminal ubiquitin-like (UbL) domain of EloB could significantly improve the stability of Vif/VifΔSLQ/VifΔPPL through the region between ...
All lentiviruses except equine infectious anemia virus (EIVA) antagonize antiviral family APOBEC3 (A3) proteins of the host through viral Vif proteins. The mechanism by which Vif of human, simian or feline immunodeficiency viruses (HIV/SIV/FIV) suppresse
The production and use of derivatives and analogs related to Vif are within the scope of the present invention. In a specific embodiment, the derivative or analog is an antagonist capable of interactively binding Vif but incapable of exhibiting the functional activities associated with a full-length...
International Journal of Nanomedicine Dovepress open access to scientific and medical research Open Access Full Text Article O r i g in a l R e s e a r c h EpoGlFydRo-tpaarmgeinteedmduelltivifeurnyctoiof nDaOl nXa-nlooacdoemdpFoes3iOte4s@for MRI and antitumor chemo-photothermal ...